Vaughn Melonie, Powell Susan, Risbrough Victoria, Zhou Xianjin
Department of Psychiatry, University of California San Diego, La Jolla, California, CA 92093, United States of America.
Neuroscience Graduate Program, University of California San Diego, La Jolla, California, CA 92093, United States of America.
bioRxiv. 2024 Apr 15:2024.04.11.589086. doi: 10.1101/2024.04.11.589086.
High titers of anti-NMDAR1 autoantibodies in human brain cause anti-NMDAR1 encephalitis, a rare disease that displays a variety of psychiatric symptoms and neurological symptoms. Currently, immunohistochemical staining and cell-based assays are the standard methods for detection and semi-quantification of the anti-NMDAR1 autoantibodies. Low titers of blood circulating anti-NMDAR1 autoantibodies have been reported in a significant subset of the general human population. However, detection and quantification of these low titers of blood circulating anti-NMDAR1 autoantibodies are problematic because of high non-specific background from less diluted serum/plasma. Development of a new method to quantify these low titers of blood anti-NMDAR1 autoantibodies is necessary to understand their potential impacts on psychiatric symptoms and cognition. Based on our previous One-Step assay, we report the development of a novel simple immunoassay to quantify cross-species blood anti-NMDAR1 autoantibodies, and its validation with immunohistochemistry and cell-based assays in both humans and mice.
人脑中高滴度的抗NMDAR1自身抗体可引发抗NMDAR1脑炎，这是一种罕见疾病，表现出多种精神症状和神经症状。目前，免疫组织化学染色和基于细胞的检测方法是检测和半定量抗NMDAR1自身抗体的标准方法。在普通人群的一个重要亚组中，已报告有低滴度的循环血液抗NMDAR1自身抗体。然而，由于未充分稀释的血清/血浆中非特异性背景较高，检测和定量这些低滴度的循环血液抗NMDAR1自身抗体存在问题。开发一种新方法来定量这些低滴度的血液抗NMDAR1自身抗体对于了解它们对精神症状和认知的潜在影响是必要的。基于我们之前的一步检测法，我们报告了一种新型简单免疫检测法的开发，用于定量跨物种血液中的抗NMDAR1自身抗体，并在人和小鼠中通过免疫组织化学和基于细胞的检测法对其进行验证。
