Cemiloglu Meryem, Ozcan Oguzhan, Kimyon Gezmis, Arpaci Abdullah, Oguzman Hamdi
Department of Molecular Biochemistry and Genetics, Health Science Institute, Hatay Mustafa Kemal University, Antakya, Hatay, Turkey.
Department of Biochemistry, Faculty of Medicine, Hatay Mustafa Kemal University, Antakya, Hatay, Turkey.
J Clin Lab Anal. 2025 Apr;39(8):e70027. doi: 10.1002/jcla.70027. Epub 2025 Apr 4.
Familial Mediterranean Fever (FMF) is an autoinflammatory disease. We aimed to investigate serum soluble Triggering Receptor Expressed on Myeloid Cells-1 and 2 (sTREM-1 and sTREM-2) levels in patients with FMF during both attack and attack-free periods and their relationship with disease activity.
Fifty-seven FMF patients, 27 in the attack and 30 in the attack-free period, along with 30 age- and sex-matched healthy controls, were enrolled in the study. Demographic and clinical data were obtained from hospital records, and the disease severity scores (DSS) were calculated. Serum levels of sTREM-1, sTREM-2, TNF-α, and IL-1β were assayed by ELISA. CRP levels were measured by the nephelometric method. Receiver operating characteristic (ROC) analysis was performed for sTREM-1 levels to detect attacks in patients with FMF.
sTREM-1 levels were significantly higher in the attack group than in the attack-free and control groups (p < 0.001). IL-1β levels were elevated in FMF patients (p = 0.003), and CRP levels differed significantly among the groups (p < 0.001). No significant differences in sTREM-2 or TNF-α were observed. In the FMF-attack group, sTREM-1 positively correlated with TNF-α (r = 0.526, p = 0.005), IL-1β (r = 0.475, p = 0.014), CRP, and fibrinogen (p < 0.001). The DSS was significantly correlated with sTREM-1 and CRP levels in FMF patients (respectively, r = 0.270, p = 0.042; r = 0.292, p = 0.027). The area under the curve (AUC) was 0.807 (95% CI, 0.695-0.92, p < 0.001), with an optimal sTREM-1 cutoff of 232 pg/mL to detect attacks.
sTREM-1 may play a critical role in the inflammatory response in FMF disease and could serve as a potential marker for assessing disease activity.
家族性地中海热（FMF）是一种自身炎症性疾病。我们旨在研究FMF患者在发作期和非发作期血清可溶性髓系细胞触发受体-1和-2（sTREM-1和sTREM-2）水平及其与疾病活动度的关系。
本研究纳入了57例FMF患者，其中发作期27例，非发作期30例，以及30例年龄和性别匹配的健康对照者。从医院记录中获取人口统计学和临床数据，并计算疾病严重程度评分（DSS）。采用酶联免疫吸附测定法（ELISA）检测血清sTREM-1、sTREM-2、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。采用散射比浊法测量C反应蛋白（CRP）水平。对sTREM-1水平进行受试者操作特征（ROC）分析，以检测FMF患者的发作情况。
发作组的sTREM-1水平显著高于非发作组和对照组（p<0.001）。FMF患者的IL-1β水平升高（p=0.003），且各组间CRP水平差异有统计学意义（p<0.001）。未观察到sTREM-2或TNF-α有显著差异。在FMF发作组中，sTREM-1与TNF-α（r=0.526，p=0.005）、IL-1β（r=0.475，p=0.014）、CRP和纤维蛋白原呈正相关（p<0.001）。FMF患者的DSS与sTREM-1和CRP水平显著相关（分别为r=0.27 , p=0.042；r=0.292，p=0.027）。曲线下面积（AUC）为0.807（95%CI，0.695-0.92，p<0.001），检测发作的最佳sTREM-1截断值为232 pg/mL。
sTREM-1可能在FMF疾病的炎症反应中起关键作用，并可作为评估疾病活动度的潜在标志物。
