Department of Internal Medicine, Ankara Bilkent City Hospital, Ankara, Turkey.
Department of Internal Medicine, Division of Rheumatology, Ankara Bilkent City Hospital, Ankara, Turkey.
Ir J Med Sci. 2024 Aug;193(4):1901-1909. doi: 10.1007/s11845-024-03659-9. Epub 2024 Mar 13.
BACKGROUND/AIM: The aim of the study was to evaluate serum calprotectin (CLP) levels in familial Mediterranean fever (FMF) patients and to investigate the utility of CLP in distinguishing patients with attack from patients without attack.
FMF patients, rheumatoid arthritis (RA) patients, and healthy controls were included. Serum calprotectin levels were quantified utilizing the enzyme-linked immunosorbent assay (ELISA) method. Receiver operating characteristic (ROC) curve analysis was used to identify the cut-off value of serum CLP level to differentiate FMF patients with attack from those without. Logistic regression analysis was performed to identify predictors.
Significant differences were observed among the three groups concerning white blood cell (WBC), neutrophil, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and serum CLP levels (p = 0.003, p = 0.004, p < 0.001, p < 0.001, and p = 0.002, respectively). Higher ESR, CRP, and serum CLP levels were observed in FMF patients with attacks than those without (all, p < 0.001). Serum CLP was significantly higher in RA patients than in FMF patients in remission (p < 0.001). ROC analysis identified a threshold CLP concentration in FMF with an attack to be 47.1 pg/mL (83.3% sensitivity, 60.6% specificity, AUC = 0.74, 95% CI = 0.63-0.85, p < 0.001). In univariate logistic regression analysis, CLP (β = 1.045, 95% CI = 1.017-1.073, p = 0.001) was predictive of FMF patients experiencing an attack.
Serum CLP proves to be as productive as ESR in illustrating inflammation and demonstrating the existence of attacks in FMF patients.
背景/目的：本研究旨在评估家族性地中海热（FMF）患者的血清钙卫蛋白（CLP）水平，并探讨 CLP 在区分发作患者和无发作患者中的作用。
纳入 FMF 患者、类风湿关节炎（RA）患者和健康对照者。采用酶联免疫吸附测定（ELISA）法定量血清钙卫蛋白水平。采用受试者工作特征（ROC）曲线分析确定区分 FMF 发作患者和无发作患者的血清 CLP 水平的临界值。采用 logistic 回归分析确定预测因子。
三组间白细胞（WBC）、中性粒细胞、红细胞沉降率（ESR）、C 反应蛋白（CRP）和血清 CLP 水平存在显著差异（p = 0.003、p = 0.004、p < 0.001、p < 0.001 和 p = 0.002）。发作的 FMF 患者的 ESR、CRP 和血清 CLP 水平均高于无发作的患者（均 p < 0.001）。RA 患者的血清 CLP 水平明显高于缓解期的 FMF 患者（p < 0.001）。ROC 分析确定 FMF 发作时的 CLP 浓度阈值为 47.1 pg/mL（83.3%的敏感性、60.6%的特异性、AUC = 0.74、95%CI = 0.63-0.85、p < 0.001）。在单因素 logistic 回归分析中，CLP（β = 1.045、95%CI = 1.017-1.073、p = 0.001）是预测 FMF 患者发作的指标。
血清 CLP 与 ESR 一样，可有效反映 FMF 患者的炎症程度，并显示患者的发作情况。
