• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人偏肺病毒A1和B1亚型半定量微量中和试验的设计与验证

Design and validation of a semi-quantitative microneutralization assay for human Metapneumovirus A1 and B1 subtypes.

作者信息

Riolo Giulia, Biagini Valentina, Guerrini Noemi, Roscia Giulia, Antonelli Roberta, Giglioli Ginevra, Stincarelli Maria Alfreda, Piu Pietro, Bonifazi Carolina, De Grazia Simona, Pizzo Mariangela, Lovreglio Piero, Stufano Angela, Trombetta Claudia Maria, Manenti Alessandro, Montomoli Emanuele, Dapporto Francesca

机构信息

VisMederi S.R.L., Siena, Italy.

Department of Life Sciences, University of Siena, Siena, Italy.

出版信息

Sci Rep. 2025 Apr 4;15(1):11614. doi: 10.1038/s41598-025-96567-6.

DOI:10.1038/s41598-025-96567-6
PMID:40185841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11971440/
Abstract

Since 2001, human Metapneumovirus has been a significant cause of human respiratory disease worldwide, and no vaccine or preventive treatment is currently available. The ELISA-based live virus microneutralization assay is a method to detect neutralizing antibodies against a target pathogen. The aim of this study was to demonstrate the suitability of this approach to quantifying neutralizing antibodies against A1 and B1 virus subtypes in human serum samples. To standardize and validate this microneutralization assay, we carried out analytical procedures according to the International Council of Harmonization guidelines; these procedures are described in detail. In addition, we compared the validated method with the indirect ELISA, and confirmed that the ELISA-based microneutralization assay provides reliable, accurate and reproducible results. The use of this high-throughput method for large-scale serological studies could effectively support the evaluation of the immunogenicity of new vaccines, thereby improving therapeutical strategies against human Metapneumovirus.

摘要

自2001年以来，人偏肺病毒一直是全球人类呼吸道疾病的一个重要病因，目前尚无疫苗或预防性治疗方法。基于酶联免疫吸附测定（ELISA）的活病毒微量中和试验是一种检测针对目标病原体的中和抗体的方法。本研究的目的是证明该方法适用于定量检测人血清样本中针对A1和B1病毒亚型的中和抗体。为了标准化和验证这种微量中和试验，我们根据国际协调理事会指南进行了分析程序；这些程序将详细描述。此外，我们将经过验证的方法与间接ELISA进行了比较，并确认基于ELISA的微量中和试验可提供可靠、准确和可重复的结果。将这种高通量方法用于大规模血清学研究可有效支持对新疫苗免疫原性的评估，从而改进针对人偏肺病毒的治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/2c8e99793dea/41598_2025_96567_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/90aee5c15d88/41598_2025_96567_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/b6345ae6fb7a/41598_2025_96567_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/1d2dc4331a66/41598_2025_96567_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/61e204b00ab8/41598_2025_96567_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/4289239013af/41598_2025_96567_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/df0d9b99bc0d/41598_2025_96567_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/2c8e99793dea/41598_2025_96567_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/90aee5c15d88/41598_2025_96567_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/b6345ae6fb7a/41598_2025_96567_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/1d2dc4331a66/41598_2025_96567_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/61e204b00ab8/41598_2025_96567_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/4289239013af/41598_2025_96567_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/df0d9b99bc0d/41598_2025_96567_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb45/11971440/2c8e99793dea/41598_2025_96567_Fig7_HTML.jpg

相似文献

1
Design and validation of a semi-quantitative microneutralization assay for human Metapneumovirus A1 and B1 subtypes.人偏肺病毒A1和B1亚型半定量微量中和试验的设计与验证
Sci Rep. 2025 Apr 4;15(1):11614. doi: 10.1038/s41598-025-96567-6.
2
Microneutralization assay for the measurement of neutralizing antibodies to human metapneumovirus.用于测量人偏肺病毒中和抗体的微量中和测定法。
J Clin Virol. 2009 Dec;46(4):314-7. doi: 10.1016/j.jcv.2009.09.020. Epub 2009 Oct 8.
3
[Analysis of neutralizing activity of sera from 0 to 6 years old children in Chongqing area against human metapneumovirus].[重庆地区0至6岁儿童血清对人偏肺病毒的中和活性分析]
Zhonghua Er Ke Za Zhi. 2007 Jun;45(6):432-6.
4
High seroprevalence of neutralizing capacity against human metapneumovirus in all age groups studied in Bonn, Germany.在德国波恩所研究的所有年龄组中，针对人偏肺病毒的中和能力血清阳性率很高。
Clin Vaccine Immunol. 2010 Mar;17(3):481-4. doi: 10.1128/CVI.00398-09. Epub 2009 Dec 30.
5
Development and validation of an enzyme-linked immunosorbent assay for human metapneumovirus serology based on a recombinant viral protein.基于重组病毒蛋白的人偏肺病毒血清学酶联免疫吸附测定法的开发与验证
Clin Diagn Lab Immunol. 2005 Feb;12(2):249-53. doi: 10.1128/CDLI.12.2.249-253.2005.
6
Development and evaluation of a whole virus-based enzyme-linked immunosorbent assay for the detection of human metapneumovirus antibodies in human sera.开发并评估了一种基于全病毒的酶联免疫吸附试验，用于检测人血清中的人类偏肺病毒抗体。
J Virol Methods. 2010 Mar;164(1-2):24-9. doi: 10.1016/j.jviromet.2009.11.019. Epub 2009 Nov 17.
7
Investigations on the protective role of passively transferred antibodies against avian metapneumovirus infection in turkeys.研究被动转移的抗禽传染性支气管炎病毒抗体对火鸡感染的保护作用。
Avian Pathol. 2009 Dec;38(6):427-36. doi: 10.1080/03079450903349204.
8
Development of A recombinant nucleocapsid based indirect ELISA for the detection of antibodies to avian metapneumovirus subtypes, A, B, and C.一种基于重组核衣壳蛋白的间接 ELISA 检测禽偏肺病毒 A、B、C 亚型抗体的建立。
Vet Immunol Immunopathol. 2021 Jan;231:110151. doi: 10.1016/j.vetimm.2020.110151. Epub 2020 Nov 11.
9
Induction of local and systemic immune reactions following infection of turkeys with avian Metapneumovirus (aMPV) subtypes A and B.火鸡感染禽偏肺病毒（aMPV）A 型和 B 型后局部和全身免疫反应的诱导
Vet Immunol Immunopathol. 2007 Feb 15;115(3-4):273-85. doi: 10.1016/j.vetimm.2006.12.001. Epub 2006 Dec 9.
10
Lung CD8+ T Cell Impairment Occurs during Human Metapneumovirus Infection despite Virus-Like Particle Induction of Functional CD8+ T Cells.尽管人偏肺病毒感染期间可诱导产生功能性CD8+ T细胞，但肺部CD8+ T细胞仍会出现功能损伤。
J Virol. 2015 Sep;89(17):8713-26. doi: 10.1128/JVI.00670-15. Epub 2015 Jun 10.

引用本文的文献

1
Immunological insights into the re-emergence of human metapneumovirus.对人偏肺病毒再次出现的免疫学见解。
Curr Opin Immunol. 2025 Jun;94:102562. doi: 10.1016/j.coi.2025.102562. Epub 2025 May 12.

本文引用的文献

1
Neutralising antibodies against human metapneumovirus.抗人偏肺病毒的中和抗体。
Lancet Microbe. 2023 Sep;4(9):e732-e744. doi: 10.1016/S2666-5247(23)00134-9. Epub 2023 Jul 24.
2
Haemagglutination inhibition and virus microneutralisation serology assays: use of harmonised protocols and biological standards in seasonal influenza serology testing and their impact on inter-laboratory variation and assay correlation: A FLUCOP collaborative study.血凝抑制和病毒微量中和血清学检测：在季节性流感血清学检测中使用协调一致的方案和生物标准及其对实验室间变异和检测相关性的影响：FLUCOP 合作研究。
Front Immunol. 2023 Apr 18;14:1155552. doi: 10.3389/fimmu.2023.1155552. eCollection 2023.
3
Antibodies to combat viral infections: development strategies and progress.
针对病毒感染的抗体：开发策略与进展。
Nat Rev Drug Discov. 2022 Sep;21(9):676-696. doi: 10.1038/s41573-022-00495-3. Epub 2022 Jun 20.
4
Profiling of hMPV F-specific antibodies isolated from human memory B cells.人记忆 B 细胞中分离的 hMPV F 特异性抗体的分析。
Nat Commun. 2022 May 10;13(1):2546. doi: 10.1038/s41467-022-30205-x.
5
Structure-based design of prefusion-stabilized human metapneumovirus fusion proteins.基于结构的人偏肺病毒融合蛋白前融合稳定构象设计。
Nat Commun. 2022 Mar 14;13(1):1299. doi: 10.1038/s41467-022-28931-3.
6
Assay Harmonization and Use of Biological Standards To Improve the Reproducibility of the Hemagglutination Inhibition Assay: a FLUCOP Collaborative Study.测定均一化和生物标准品的使用以提高血凝抑制试验的重现性：FLUCOP 协作研究。
mSphere. 2021 Aug 25;6(4):e0056721. doi: 10.1128/mSphere.00567-21. Epub 2021 Jul 28.
7
Antibody recognition of the Pneumovirus fusion protein trimer interface.呼吸道合胞病毒融合蛋白三聚体界面的抗体识别。
PLoS Pathog. 2020 Oct 9;16(10):e1008942. doi: 10.1371/journal.ppat.1008942. eCollection 2020 Oct.
8
A Potent Neutralizing Site III-Specific Human Antibody Neutralizes Human Metapneumovirus .一种强效的中和位点 III 特异性人源抗体中和人类偏肺病毒。
J Virol. 2019 Sep 12;93(19). doi: 10.1128/JVI.00342-19. Print 2019 Oct 1.
9
Human Metapneumovirus: Laboratory Methods for Isolation, Propagation, and Plaque Titration.人偏肺病毒：分离、增殖和噬斑滴定的实验室方法。
Intervirology. 2018;61(6):301-306. doi: 10.1159/000497309. Epub 2019 Mar 27.
10
Human Metapneumovirus: Mechanisms and Molecular Targets Used by the Virus to Avoid the Immune System.人偏肺病毒：病毒逃避免疫系统的机制和分子靶标。
Front Immunol. 2018 Oct 24;9:2466. doi: 10.3389/fimmu.2018.02466. eCollection 2018.