Hankin L, Anagnostakis S L
J Gen Microbiol. 1977 Jan;98(1):109-15. doi: 10.1099/00221287-98-1-109.
Solid media containing carboxymethyl cellulose (CMC) were developed to detect CX cellulose-producing micro-organisms. Hydrolysis of CMC was seen as a clear zone around colonies after flooding plates with 1% aqueous hexadecyltrimethyl-ammonium bromide. Tests with ten bacterial and four fungal species showed that the degree of substitution (DS) of the CMC affects both growth and enzyme production. Most of the organisms produced more CX cellulase on CMC with a DS of 0-9, but CMC with a DS of 0-4 was better for one fungus. A qualitative measure of cellulase production may be obtained by calculating the ratio of zone size to colony diameter. Solid media containing CMC provided a more rapid assay of CX cellulose production than a medium containing native cellulose.
开发了含有羧甲基纤维素（CMC）的固体培养基来检测产生CX纤维素的微生物。在用1%十六烷基三甲基溴化铵水溶液覆盖平板后，CMC的水解表现为菌落周围的清晰区域。对十种细菌和四种真菌的测试表明，CMC的取代度（DS）会影响生长和酶的产生。大多数微生物在DS为0-9的CMC上产生更多的CX纤维素酶，但DS为0-4的CMC对一种真菌更好。通过计算区域大小与菌落直径的比率，可以获得纤维素酶产生的定性测量。与含有天然纤维素的培养基相比，含有CMC的固体培养基提供了一种更快的CX纤维素产生检测方法。
