Roussos S, Raimbault M
Ann Microbiol (Paris). 1982 Nov-Dec;133(3):455-64.
Trichoderma harzianum was selected from 30 strains of cellulolytic fungi with the aim of producing cellulases by solid state fermentation of lignocellulosic substrates. Special attention was paid to cellulase production (i.e. carboxymethylcellulase and filter paper activity), apical growth and conidia production. Under the conditions of our experiments, T. harzianum exhibited the highest cellulasic activities with 1,315 IU/l of carboxymethyl cellulose and 80 IU/l of filter paper activity. Apical growth (1 mm/h) and yield of conidial production (3.25 x 10(10) conidia/g of substrate dry weight) were also valuable characteristics of this strain in the use of solid state fermentation.
从30株纤维素分解真菌中筛选出哈茨木霉,目的是通过木质纤维素底物的固态发酵生产纤维素酶。特别关注纤维素酶的产生(即羧甲基纤维素酶和滤纸酶活性)、顶端生长和分生孢子的产生。在我们的实验条件下,哈茨木霉表现出最高的纤维素酶活性,羧甲基纤维素酶活性为1315 IU/l,滤纸酶活性为80 IU/l。顶端生长速度(1毫米/小时)和分生孢子产量(3.25×10¹⁰个分生孢子/克底物干重)也是该菌株在固态发酵应用中的重要特性。