Pinkerton C R, Booth I W, Milla P J
Gut. 1985 Jul;26(7):704-9. doi: 10.1136/gut.26.7.704.
The topical effect of methotrexate (MTX) on small intestinal hexose and ion transport has been studied using an in vivo steady state jejunal perfusion technique in the rat, and short circuited rabbit terminal ileum in Ussing chambers in vitro. In rat jejunum, perfusion with MTX (1 mumol/l) caused significant reductions in water, sodium, and glucose absorption within 110 minutes of exposure. Fructose absorption was, however, unimpaired. The same concentration of MTX, when added to the mucosal side of distal rabbit ileum caused significant increases in transmucosal potential difference, short circuit current and the unidirectional flux of chloride from serosa to mucosa. In the presence of a subphysiological magnesium concentration (0.3 mmol/l), MTX resulted in the abolition of net sodium absorption and the conversion of net chloride absorption to secretion. We conclude that MTX has a topical effect on small intestinal transport which is independent of its effect on crypt cell kinetics.
利用大鼠体内稳态空肠灌注技术以及体外乌斯电极小室中短路的兔回肠末端，研究了甲氨蝶呤（MTX）对小肠己糖和离子转运的局部作用。在大鼠空肠中，灌注MTX（1μmol/L）在暴露110分钟内导致水、钠和葡萄糖吸收显著减少。然而，果糖吸收未受影响。当将相同浓度的MTX添加到兔回肠远端的黏膜侧时，跨黏膜电位差、短路电流以及氯离子从浆膜到黏膜的单向通量显著增加。在亚生理镁浓度（0.3mmol/L）存在的情况下，MTX导致净钠吸收消失，并使净氯吸收转变为分泌。我们得出结论，MTX对小肠转运有局部作用，这与其对隐窝细胞动力学的作用无关。
