Kang Hyun Mi, Chu Jiyon, Yoo In Hyuk, Yoo In Young, Shin Jeong-Ih, Seo Mi-Ran, Chung Yeun-Jun, Jung Seung-Hyun, Park Yeon Joon
Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Vaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Ann Lab Med. 2025 May 1;45(3):312-321. doi: 10.3343/alm.2024.0378. Epub 2025 Apr 4.
Understanding the virulence and pathogenicity of invasive nontyphoidal (iNTS) in children may support timely treatment and enable closer monitoring of chronic infections. iNTS epidemiology in Asia remains inadequately described. We analyzed the genetic diversity and virulence genes associated with extra-intestinal invasion in Korean children.
Salmonella isolates from children <18 yrs of age diagnosed with moderate-to-severe salmonellosis between January 2019 and December 2021 were subjected to antibiotic susceptibility testing and whole-genome sequencing.
In total, 58 cases were included. We identified 20 serotypes, the most prevalent being Enteritidis (N=21), followed by Infantis (N=6), I 4,[5],12:i:- (N=5), and Bareilly (N=5). Extra-intestinal invasion occurred in 12 (20.7%) cases involving Oranienburg (2/2), Give (1/1), Javiana (1/1), Paratyphi B var. L(+) tartrate+ (1/1), Schwarzengrund (1/1), Singapore (1/1), Montevideo (1/2), Saintpaul (1/2), I 4:b:- (1/2), Infantis (1/6), and Enteritidis (1/21). While the numbers of total virulence genes and genes belonging to major virulence categories did not significantly differ between iNTS and non-iNTS, several genetic factors, including pathogenicity island (SPI)-1 ( =0.039), SPI-2 ( =0.020), SPI-5 ( =0.014), SPI-13 ( =0.010), cytolethal distending toxin-related genes ( =1.4×10), ( =0.021), and ( =0.040) were more frequent in invasive isolates.
Enteritidis-ST11 predominated in infections among Korean children, but invasive isolates were rare. Early detection of genetic factors associated with extra-intestinal invasion will be helpful for prompt and appropriate treatment.
了解儿童侵袭性非伤寒沙门氏菌（iNTS）的毒力和致病性有助于及时治疗，并能更密切地监测慢性感染情况。亚洲iNTS的流行病学情况仍描述不足。我们分析了韩国儿童中与肠外侵袭相关的基因多样性和毒力基因。
对2019年1月至2021年12月期间诊断为中重度沙门氏菌病的18岁以下儿童的沙门氏菌分离株进行药敏试验和全基因组测序。
共纳入58例病例。我们鉴定出20种血清型，最常见的是肠炎沙门氏菌（N = 21），其次是婴儿沙门氏菌（N = 6）、I 4,[5],12:i:-（N = 5）和巴雷利沙门氏菌（N = 5）。12例（20.7%）发生了肠外侵袭，涉及奥兰宁堡沙门氏菌（2/2）、吉夫沙门氏菌（1/1）、哈维亚纳沙门氏菌（1/1）、副伤寒乙变种L(+)酒石酸盐阳性沙门氏菌（1/1）、施瓦岑格伦德沙门氏菌（1/1）、新加坡沙门氏菌（1/1）、蒙得维的亚沙门氏菌（1/2）、圣保罗沙门氏菌（1/2）、I 4:b:-（1/2）、婴儿沙门氏菌（1/6）和肠炎沙门氏菌（1/21）。虽然iNTS和非iNTS之间的总毒力基因数量以及属于主要毒力类别的基因数量没有显著差异，但一些遗传因素，包括致病岛（SPI）-1（P = 0.039）、SPI-2（P = 0.020）、SPI-5（P = 0.014）、SPI-13（P = 0.010）、细胞致死扩张毒素相关基因（P = 1.4×10）、（P = 0.021）和（P = 0.040）在侵袭性分离株中更为常见。
肠炎沙门氏菌-ST11在韩国儿童感染中占主导地位，但侵袭性分离株很少见。早期检测与肠外侵袭相关的遗传因素将有助于及时进行适当治疗。
