Lee Solpa, Kim Hyunsoo, Kim Minwoo, Kang Ryun, Lim Inje, Jang Yongwoo
Department of Medical and Digital Engineering, College of Engineering, Hanyang University, Seoul, 04763, Korea.
DRB Research, DRB Industrial, 28, Gongdandong-ro 55beon-gil, Busan, 46329, Republic of Korea.
J Nanobiotechnology. 2025 Apr 7;23(1):279. doi: 10.1186/s12951-025-03341-x.
Salmonella is a major foodborne pathogen that causes salmonellosis, which is characterized by symptoms such as diarrhea, fever, and abdominal cramps. Existing methods for detecting Salmonella, such as culture plating, ELISA, and PCR, are accurate but time-consuming and unsuitable for on-site applications. In this study, we developed a rapid and sensitive electrochemical sensor using a molecularly imprinted polymer (MIP) to detect Salmonella typhimurium (S. typhimurium) by targeting lipopolysaccharides (LPS). Polydopamine (PDA) was used as the polymer matrix because of its cost-efficiency and functional versatility. The sensor demonstrated high sensitivity and selectivity, with a detection limit of 10 CFU/mL and a linear response over the 10²-10⁸ CFU/mL range. The specificity of the sensor was validated against other gram-positive and gram-negative bacteria and showed no significant cross-reactivity. Furthermore, the sensor performed effectively in real food samples, including tap water, milk, and pork, without complex preprocessing. These results highlight the potential of the LPS-imprinted MIP sensor for practical on-site detection of S. typhimurium, improving food safety monitoring and preventing outbreaks in food-handling environments.
沙门氏菌是一种主要的食源性病原体，可引起沙门氏菌病，其症状包括腹泻、发烧和腹部绞痛。现有的检测沙门氏菌的方法，如培养平板法、酶联免疫吸附测定法（ELISA）和聚合酶链反应（PCR），虽然准确，但耗时且不适用于现场应用。在本研究中，我们开发了一种快速灵敏的电化学传感器，该传感器使用分子印迹聚合物（MIP）通过靶向脂多糖（LPS）来检测鼠伤寒沙门氏菌（S. typhimurium）。由于聚多巴胺（PDA）具有成本效益和功能多样性，因此被用作聚合物基质。该传感器表现出高灵敏度和选择性，检测限为10 CFU/mL，在10²-10⁸ CFU/mL范围内呈线性响应。该传感器针对其他革兰氏阳性菌和革兰氏阴性菌验证了其特异性，未显示出明显的交叉反应。此外，该传感器在包括自来水、牛奶和猪肉在内的实际食品样品中无需复杂的预处理即可有效运行。这些结果突出了LPS印迹MIP传感器在实际现场检测鼠伤寒沙门氏菌方面的潜力，可改善食品安全监测并预防食品处理环境中的疫情爆发。
