Bendjellit S, Mathé D, Castaing D, Bismuth H, Lutton C
Int J Obes. 1985;9(1):1-9.
The effects of portacaval anastomosis (PCA) on cholesterol biodynamics of male adult (fa/fa) Zucker rats and their lean littermates were studied with an isotopic equilibrium method. Animals were fed with a sucrose-rich semi-purified diet. Obese rats were hypercholesterolemic (2.03 +/- 0.14 vs 1.06 +/- 0.7 mg/ml), had a cholesterol-enriched liver (135.3 +/- 14.5 vs 40.0 +/- 2.6 mg/liver) and accumulated cholesterol in body pools. However no difference in the rates of cholesterol absorption, synthesis, fecal elimination or transformation into bile acids distinguished obese from lean Zucker rats. In both lean and obese rats, PCA decreased cholesterolemia by about 28 per cent and liver weight by 40 per cent while the total cholesterol content of the liver was not affected. Input of synthesized cholesterol (internal secretion) was strikingly decreased by the shunt (from 13.2 +/- 0.6 and 12.6 +/- 0.7 mg/day/rat before PCA, to 8.9 +/- 0.8 and 8.6 +/- 1.0 mg/day/rat after PCA) in lean and obese rats respectively. A similar decrease was observed in the cholesterol transformation into bile acids. Since the activity of the gut for cholesterol synthesis, as shown by the fecal external secretion (cholesterol synthesized by the gut and directly eliminated in the gut and feces) was probably not modified, the reduction of internal secretion induced by PCA resulted from decreased hepatic cholesterogenesis. It is suggested that this decrease may be one of the factors involved in the lowering effect of PCA on plasma cholesterol level.
采用同位素平衡法研究了门腔静脉吻合术（PCA）对成年雄性（fa/fa） Zucker肥胖大鼠及其瘦同胞大鼠胆固醇生物动力学的影响。给动物喂食富含蔗糖的半纯化饮食。肥胖大鼠存在高胆固醇血症（2.03±0.14 vs 1.06±0.7mg/ml），肝脏胆固醇含量丰富（135.3±14.5 vs 40.0±2.6mg/肝脏），且胆固醇在体内蓄积。然而，肥胖 Zucker大鼠与瘦 Zucker大鼠在胆固醇吸收、合成、粪便排泄或转化为胆汁酸的速率方面并无差异。在瘦大鼠和肥胖大鼠中，PCA均可使胆固醇血症降低约28%，肝脏重量降低40%，而肝脏总胆固醇含量不受影响。PCA分流术使瘦大鼠和肥胖大鼠合成胆固醇的输入量（内分泌）显著降低（PCA前分别为13.2±0.6和12.6±0.7mg/天/大鼠，PCA后分别为8.9±0.8和8.6±1.0mg/天/大鼠）。在胆固醇转化为胆汁酸方面也观察到类似的降低。由于肠道胆固醇合成活性（如粪便外分泌所示，即肠道合成并直接在肠道和粪便中排泄的胆固醇）可能未发生改变，PCA诱导的内分泌减少是由于肝脏胆固醇生成减少所致。提示这种降低可能是PCA降低血浆胆固醇水平作用的相关因素之一。
