Nabi Ali, Dehnavi Azam Hassanpour, Mazaheri Fahime, Momeni Nastaran, Nikukar Habib, Hemati Mahdie, Hosseinimehr Seyed Jalal, Shabani Masoud, Sadeghian-Nodoushan Fatemeh, Biabani-Ardakani Javad
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Int J Reprod Biomed. 2025 Mar 21;23(1):91-102. doi: 10.18502/ijrm.v23i1.18202. eCollection 2025 Jan.
Radiation-induced normal tissue damage remains a major concern in radiotherapy, particularly affecting rapidly dividing cells, including those in reproductive tissues. Developing effective radioprotective agents to mitigate this damage is crucial for preserving fertility.
The radioprotective effects of omeprazole (OMP) were investigated in adult male mice undergoing external radiation.
In this experimental study, 36 adult male mice (30-35 gr, 6-8 wk old) were divided into 6 groups and orally administered OMP daily via oral gavage for 7 days before whole-body irradiation. On the 8 day, mice were subjected to a single 6 Gray dose of 6 megavoltage X-ray radiation. Blood samples were collected via cardiac puncture for testosterone level evaluation, while testicular specimens were harvested post-euthanasia for sperm parameters assessment and histological analysis. Additionally, spermatogenic cell density was evaluated.
Irradiation of 6 Gray X-ray to the testis of mice significantly affected sperm count, progressive motility, DNA fragmentation, the number of sperm with normal morphology, and the number of immotile sperm. Furthermore, administration of OMP improved progressive motility, DNA fragmentation, and sperm viability. Histopathological findings showed irradiation led to severe testicular atrophy with spermatogenic arrest and abnormal cytoarchitecture vacuolation and interstitial edema, while OMP treatment reversed relative radiation toxicity, especially in the 50 mg OMP treatment group.
In conclusion, OMP could act as an effective radioprotector against testicular damage following X-ray irradiation in an animal model. Further studies are needed to investigate OMP potential in protecting human testis tissue.
辐射引起的正常组织损伤仍是放射治疗中的一个主要问题，尤其会影响快速分裂的细胞，包括生殖组织中的细胞。开发有效的辐射防护剂以减轻这种损伤对于保护生育能力至关重要。
研究奥美拉唑（OMP）对接受外照射的成年雄性小鼠的辐射防护作用。
在本实验研究中，将36只成年雄性小鼠（30 - 35克，6 - 8周龄）分为6组，在全身照射前7天通过灌胃法每日口服给予OMP。在第8天，小鼠接受单次6格雷剂量的6兆伏X射线辐射。通过心脏穿刺采集血样以评估睾酮水平，在安乐死后采集睾丸标本以评估精子参数并进行组织学分析。此外，评估生精细胞密度。
对小鼠睾丸进行6格雷X射线照射显著影响精子数量、前向运动能力、DNA碎片化、正常形态精子数量和不动精子数量。此外，给予OMP可改善前向运动能力、DNA碎片化和精子活力。组织病理学结果显示，照射导致严重的睾丸萎缩，伴有生精停滞、细胞结构异常空泡化和间质水肿，而OMP治疗可逆转相对辐射毒性，尤其是在50毫克OMP治疗组。
总之，在动物模型中，OMP可作为一种有效的辐射防护剂，防止X射线照射后对睾丸的损伤。需要进一步研究以调查OMP在保护人类睾丸组织方面的潜力。
