α-生育酚醋酸酯的立体异构体。III. 同时测定大鼠的吸收-妊娠和肌病，以此作为评估消旋-α-生育酚醋酸酯和RRR-α-生育酚醋酸酯生物效价比的一种方法。

Stereoisomers of alpha-tocopheryl acetate. III. Simultaneous determination of resorption-gestation and myopathy in rats as a means of evaluating biopotency ratios of all-rac- and RRR-alpha-tocopheryl acetate.

作者信息

Weiser H, Vecchi M, Schlachter M

出版信息

Int J Vitam Nutr Res. 1985;55(2):149-58.

PMID:4019069

Abstract

The resorption-gestation test furnishes information on a more complex vitamin activity than is the case with other vitamin E tests, so that correspondingly more importance attaches to the results. A drawback is their questionable validity for species other than the small rodents in which fetal resorption has so far been observed. In the four experiments carried out, RRR-alpha-tocopheryl acetate (alpha-TA) was compared with all-rac-alpha-TA (USP standard) and a mean relative activity of 1.32 was determined. The additional measurement of pyruvate kinase (PK) activity serves to emphasize the correlation with nutrition-linked myopathy. This biochemical lesion is seen in various animal species and man. In two experiments, the parameters fetal resorption and plasma PK activity were measured together. For all-rac-alpha-TA and RRR-alpha-TA, the following activity ratios were calculated: Resorption-gestation 1:1.35; Myopathy 1:1.47. It could be shown that the two parameters are measurable quantitatively in a single experimental set-up and that the results are in good agreement. The accepted activity of 1 mg RRR-alpha-TA = 1.36 USP Units was fully confirmed.

摘要

再吸收-妊娠试验所提供的关于维生素活性的信息，比其他维生素E试验所涉及的更为复杂，因此相应地，其结果也更为重要。一个缺点是，对于除小型啮齿动物之外的其他物种，该试验的有效性存疑，因为到目前为止，仅在小型啮齿动物中观察到胎儿再吸收现象。在进行的四项实验中，将RRR-α-生育酚乙酸酯（α-TA）与消旋-α-TA（USP标准品）进行了比较，并测定了平均相对活性为1.32。丙酮酸激酶（PK）活性的额外测定有助于强调与营养相关的肌病之间的相关性。这种生化损伤在多种动物物种和人类中都有发现。在两项实验中，同时测量了胎儿再吸收和血浆PK活性这两个参数。对于消旋-α-TA和RRR-α-TA，计算出了以下活性比：再吸收-妊娠为1:1.35；肌病为1:1.47。结果表明，这两个参数可以在单一实验设置中进行定量测量，且结果吻合良好。1 mg RRR-α-TA = 1.36 USP单位这一公认的活性得到了充分证实。