通过大鼠治疗性肌病生物测定法测定的α-生育酚的生物效价。
Biopotency of alpha-tocopherols as determined by curative myopathy bioassay in the rat.
作者信息
Machlin L J, Gabriel E, Brin M
出版信息
J Nutr. 1982 Jul;112(7):1437-40. doi: 10.1093/jn/112.7.1437.
The decrease in plasma pyruvate kinase activity after administration of various tocopherols to vitamin E-deficient rats provided the basis for a 3-point parallel-slope assay for vitamin E activity. Based on 6 replicate assays, RRR-alpha-tocopheryl acetate (RRR-alpha-TA) was 141% as active as all-rac-alpha-tocopheryl acetate (all-rac-alpha-TA). This confirms many previous reports with other bioassays and is in agreement with the unit-weight relationships assigned by The National Formulary. 2-ambo-alpha-Tocopheryl acetate was 97% as active as all-rac-alpha-TA indicating that only the configuration of the 2-position on the chromanol ring is important in determining the biological activity of alpha-tocopherol.
给维生素E缺乏的大鼠施用各种生育酚后，血浆丙酮酸激酶活性降低，这为维生素E活性的三点平行斜率测定提供了基础。基于6次重复测定，RRR-α-生育酚醋酸酯（RRR-α-TA）的活性是消旋-α-生育酚醋酸酯（消旋-α-TA）的141%。这证实了许多先前其他生物测定的报告，并且与美国国家处方集规定的单位重量关系一致。2-消旋-α-生育酚醋酸酯的活性是消旋-α-TA的97%，这表明仅色满醇环上2位的构型在决定α-生育酚的生物活性方面很重要。
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