Khesali Fatemeh, Yousefi Azizollah, Ahmadi Seyyed Amir Yasin, Nekouian Reza
Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Pediatrics, Rasoul Akram Complex Clinical Research Development Center (RCRDC), School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Biochem Res Int. 2025 Mar 29;2025:8879418. doi: 10.1155/bri/8879418. eCollection 2025.
According to the importance of long noncoding RNAs (LncRNA) in the pathogenesis of inflammatory bowel disease (IBD) and also the lack of study for pediatric IBD in this regard, we investigated the expression of a selected panel of LncRNAs in Iranian pediatric cases of IBD compared to adult cases and healthy samples. In this gene expression study, blood samples were taken from the three groups of pediatric IBD cases, adult IBD cases, and pediatric healthy samples (for gene expression calibration). The investigated LncRNAs were , , , and . Real-time PCR was used and fold changes (FCs) were reported. A total of 50 individuals were studied including 28 cases of pediatric IBD, 12 cases of controls, and 10 cases of adult IBD. showed upregulation in adult IBD (FC = 10.56, = 0.007). showed downregulations for pediatric IBD (FC = 0.01, < 0.001) and adult IBD (FC = 0.10, = 0.039). showed downregulation in pediatric IBD (FC < 0.01, < 0.001). CDKN2B showed upregulation in pediatric IBD (FC = 17.39, < 0.001). The results were in contrast with the literature. It seems that these LncRNAs may have different roles in pediatric IBD. Further studies are needed on pediatric cases of IBD.
鉴于长链非编码RNA（LncRNA）在炎症性肠病（IBD）发病机制中的重要性，以及在这方面缺乏针对儿童IBD的研究，我们调查了一组选定的LncRNAs在伊朗儿童IBD病例中的表达情况，并与成人病例和健康样本进行比较。在这项基因表达研究中，从三组样本中采集血样：儿童IBD病例组、成人IBD病例组和儿童健康样本组（用于基因表达校准）。所研究的LncRNAs为 、 、 和 。采用实时定量聚合酶链反应（Real-time PCR）并报告倍数变化（FCs）。总共研究了50名个体，包括28例儿童IBD病例、12例对照和10例成人IBD病例。 在成人IBD中呈上调（FC = 10.56， = 0.007）。 在儿童IBD（FC = 0.01， < 0.001）和成人IBD（FC = 0.10， = 0.039）中均呈下调。 在儿童IBD中呈下调（FC < 0.01， < 0.001）。CDKN2B在儿童IBD中呈上调（FC = 17.39， < 0.001）。这些结果与文献报道相反。似乎这些LncRNAs在儿童IBD中可能具有不同作用。需要对儿童IBD病例进行进一步研究。
