Dai Yili, Yang Meifang, Luo Weiling, Qiu Yanli, Zhou Fang, Zheng Xing, Zhao Fei, Yao Xu, Zhao Suwen, Tao Houchao
Institute of Pharmacy and Pharmacology, Hengyang Medicinal School, University of South China, Hengyang, Hunan, 421001, China.
Shanghai Frontiers Science Center of TCM Chemical Biology, Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Chemistry. 2025 May 19;31(28):e202501128. doi: 10.1002/chem.202501128. Epub 2025 Apr 21.
Detergents are essential molecular tools for membrane protein (MP) research, yet traditional detergents with static properties often fail to address the diverse and evolving needs of MP studies. To this end, this study introduces "living detergents", an innovative class of detergents equipped with functional tags that enable bioorthogonal modifications with externally introduced structural elements. This approach allows for not only the parallel generation of new detergents, but also in situ tuning of MP samples within freshly formed detergents. The efficacy of this strategy was demonstrated through the rapid identification of optimal detergents for high-quality electron microscopy studies of AAR. Overall, this flexible and robust platform enables efficient tailoring of detergents, advancing the exploration of detergent structure-function relationships in MP research and opening pathways for more specialized solutions for diverse experimental demands.
去污剂是膜蛋白（MP）研究中必不可少的分子工具，但具有静态性质的传统去污剂往往无法满足MP研究多样化且不断变化的需求。为此，本研究引入了“活性去污剂”，这是一类创新型去污剂，带有功能标签，能够与外部引入的结构元件进行生物正交修饰。这种方法不仅允许并行生成新的去污剂，还能在新形成的去污剂中原位调整MP样品。通过快速确定用于AAR高质量电子显微镜研究的最佳去污剂，证明了该策略的有效性。总体而言，这个灵活且强大的平台能够高效定制去污剂，推动MP研究中去污剂结构 - 功能关系的探索，并为满足各种实验需求的更专业解决方案开辟道路。
