Mekhoukh Nasreddine, Chougui Nadia, Vilas-Boas Ana A, Pintado Manuela, Bendif Hamdi, Zancato Mirella, Bellik Youba, Sid Nassim, Peron Gregorio
Département de Biologie Physico-Chimique, Faculté des Sciences de la Nature et de la vie, Université de Bejaia, 06000 Bejaia, Algeria.
Département des Sciences Alimentaires, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Algeria.
J Pharm Pharmacol. 2025 Jul 1;77(7):970-982. doi: 10.1093/jpp/rgaf010.
This study is the first to comprehensively investigate the phenolic profile, therapeutic potential, and acute toxicity of Putoria calabrica, a Mediterranean medicinal plant. It aims to evaluate its potential for innovative wound healing formulations by analyzing the phenolic composition of five extracts, assessing antifungal activity, and evaluating toxicity, hemoglobin oxidative status, and wound healing efficacy in animal models.
The phenolic content of the extracts was analyzed using HPLC-DAD. Antifungal activity was assessed on solid PDA media, while biochemical parameters were determined spectrophotometrically.
Ten phenolics were identified, with vitexin (20.84 mg/g), rutin (17.66 mg/g), and chlorogenic acid (14.15 mg/g) as the predominant. Methanol extract showed the highest antifungal activity against Fusarium oxysporum and Penicillium chrysogenum with rates of 57.61% and 59.62% inhibition respectively, and a Minimum Inhibitory Concentration of 8 mg/ml, comparable to ethanol extract. The latter also inhibited hemoglobin degradation and methemoglobin formation at 2.5-5.0 mg/ml. In mice, ethanol extract ointments (5% and 10%) showed no toxicity, with a 96.43% wound contraction after 18 days of applying the 10% formulation.
The current findings suggest that P. calabrica leaf extracts may offer a promising natural remedy with wound healing, antioxidant, and antifungal properties, deserving further investigation for therapeutic applications.
