Tamiya-Koizumi K, Koizumi K, Ishihara H, Kojima K
J Biochem. 1985 Mar;97(3):773-9. doi: 10.1093/oxfordjournals.jbchem.a135117.
Hyperplastic nodules and hepatomas were induced in livers of rats fed a diet containing 0.05% N-2-fluorenylacetamide (2-FAA). The lipid contents, and phospholipid and fatty acid compositions were analyzed in plasma membranes (PM's) isolated from these tissues and normal rat liver, and the following trends were observed. The molar ratio of cholesterol to phospholipid-phosphorus (phospholipid-P) increased in the order: hepatoma less than normal liver less than hyperplastic nodules. The molar percentage of plasmalogen to phospholipid-P decreased in the order: hepatoma = hyperplastic nodules greater than normal liver. The percentages of choline phosphoglycerides (sum of phosphatidylcholine and lysophosphatidylcholine) and ethanolamine phosphoglycerides (sum of phosphatidylethanolamine and lysophosphatidylethanolamine) both decreased in the order: hepatoma greater than hyperplastic nodules greater than normal liver. On the other hand, the percentages of sphingomyelin and phosphatidylserine both increased in the order: hepatoma less than hyperplastic nodules less than normal liver. As regards fatty acid composition, the percentages of both 18:1 and 18:2 decreased in the order: hepatoma greater than hyperplastic nodules greater than normal liver. Those of 18:0 and 20:4 increased in the order: hepatoma less than hyperplastic nodules less than normal liver. These results suggested that the lipid bilayer in PM of hyperplastic nodules has characteristics roughly intermediate between those of hepatoma and liver PM's, although the molar ratio of cholesterol to phospholipid-P in hyperplastic nodules PM was not intermediate.
给喂食含0.05% N - 2 - 芴基乙酰胺（2 - FAA）饮食的大鼠肝脏诱导出增生性结节和肝癌。分析了从这些组织以及正常大鼠肝脏分离出的质膜（PM）中的脂质含量、磷脂和脂肪酸组成，观察到以下趋势。胆固醇与磷脂磷（磷脂 - P）的摩尔比按以下顺序增加：肝癌＜正常肝脏＜增生性结节。缩醛磷脂与磷脂 - P的摩尔百分比按以下顺序降低：肝癌 = 增生性结节＞正常肝脏。胆碱磷酸甘油酯（磷脂酰胆碱和溶血磷脂酰胆碱之和）和乙醇胺磷酸甘油酯（磷脂酰乙醇胺和溶血磷脂酰乙醇胺之和）的百分比均按以下顺序降低：肝癌＞增生性结节＞正常肝脏。另一方面，鞘磷脂和磷脂酰丝氨酸的百分比均按以下顺序增加：肝癌＜增生性结节＜正常肝脏。关于脂肪酸组成，18:1和18:2的百分比均按以下顺序降低：肝癌＞增生性结节＞正常肝脏。18:0和20:4的百分比按以下顺序增加：肝癌＜增生性结节＜正常肝脏。这些结果表明，增生性结节质膜中的脂质双层具有大致介于肝癌和肝脏质膜之间的特征，尽管增生性结节质膜中胆固醇与磷脂 - P的摩尔比并非处于中间状态。
