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美洛昔康和左旋咪唑的施用可减轻口蹄疫疫苗接种对萨希瓦尔公牛冷冻精液特性的应激影响。

Meloxicam and levamisole administration ameliorates FMD vaccination stress on frozen semen attributes in Sahiwal bulls.

作者信息

Gupta Vinod Kumar, Mohanty Tushar Kumar, Bhakat Mukesh, Baithalu Rubina K, Kumar Nishant, Dewry Raju Kumar, Nain Dipti, Soe Aye, Arunkumar Ramasamy

机构信息

ARGO, ICAR-National Dairy Research Institute (Deemed University), Karnal, India.

LPM, ICAR-National Dairy Research Institute (Deemed University), Karnal, India.

出版信息

Sci Rep. 2025 Apr 7;15(1):11863. doi: 10.1038/s41598-024-81460-5.

DOI:10.1038/s41598-024-81460-5
PMID:40195339
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11976905/
Abstract

Vaccination-induced allergic reactions in bulls raise both body and testicular temperatures, leading to germ cell damage, epididymal dysfunction, accelerated testicular ageing, and increased sperm abnormalities that ultimately degrade semen quality. This study aimed to ameliorate vaccination stress and improve semen quality using meloxicam and levamisole. The present study was conducted at the Artificial Breeding Research Centre, ICAR-NDRI, Karnal, Haryana, with twelve Sahiwal breeding bulls that were divided into four groups: Group I (Control), Group II (Meloxicam), Group III (Levamisole), and Group IV (Meloxicam + Levamisole). Semen parameters were evaluated during pre- and post-vaccination. Treated groups showed significant improvement (p < 0.05) in initial progressive motility, live sperm, HOST, and acrosome integrity compared to the control, with the highest improvement observed in the combination group (meloxicam + levamisole). Sperm abnormalities, moribund, dead, apoptotic sperm, and lipid peroxidation were significantly higher in the control group. The meloxicam + levamisole group exhibited significantly lower (p < 0.05) sperm abnormalities and lipid peroxidation. Improvement was observed in semen quality with treatment (Meloxicam + levamisole-treated bulls) in percentage of live (+ 53.11%), moribund (- 33.83%), dead (- 36.70%), apoptotic spermatozoa (- 44.01%), and DNA fragmentation index (- 47.07%) in relation to the control groups on the 7th post vaccination day.

摘要

公牛接种疫苗引起的过敏反应会使体温和睾丸温度升高，导致生殖细胞损伤、附睾功能障碍、睾丸加速老化以及精子异常增加，最终降低精液质量。本研究旨在使用美洛昔康和左旋咪唑减轻接种疫苗的应激反应并提高精液质量。本研究在哈里亚纳邦卡纳尔市的印度农业研究委员会国家家畜研究所人工授精研究中心进行，选用了12头萨希瓦尔种公牛，分为四组：第一组（对照组）、第二组（美洛昔康组）、第三组（左旋咪唑组）和第四组（美洛昔康 + 左旋咪唑组）。在接种疫苗前后对精液参数进行评估。与对照组相比，治疗组在初始前向运动率、活精子、HOST和顶体完整性方面有显著改善（p < 0.05），联合组（美洛昔康 + 左旋咪唑）改善最为明显。对照组的精子异常、濒死、死亡、凋亡精子以及脂质过氧化水平显著更高。美洛昔康 + 左旋咪唑组的精子异常和脂质过氧化水平显著更低（p < 0.05）。在接种疫苗后第7天，与对照组相比，治疗组（美洛昔康 + 左旋咪唑处理的公牛）的精液质量在活精子百分比（提高53.11%）、濒死精子百分比（降低33.83%）、死亡精子百分比（降低36.70%）、凋亡精子百分比（降低44.01%）和DNA碎片化指数（降低47.07%）方面均有改善。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c580/11976905/9cf9c6bd216e/41598_2024_81460_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c580/11976905/a74eeb23f31e/41598_2024_81460_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c580/11976905/4c3a02220ce4/41598_2024_81460_Fig9_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c580/11976905/db9c19de0394/41598_2024_81460_Fig12_HTML.jpg

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Bovine reproductive immunoinfertility: pathogenesis and immunotherapy.牛生殖免疫性不育：发病机制与免疫治疗
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