Wang Ya, Li Zhiguo, Zuo Zhicai, Gu Xiaobin, Cai Dongjie, Hu Jianfeng, Gu Yu, Shen Liuhong, Gou Liping, Zhang Kun, Ma Xiaoping
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, China.
Front Vet Sci. 2025 Mar 24;12:1546586. doi: 10.3389/fvets.2025.1546586. eCollection 2025.
Dermatophytosis, which is a contagious fungal skin infection common in animals and humans, is the most common skin disease in cattle. It has a serious negative impact on the livestock industry. In order to circumvent the shortcomings of traditional detection methods such as time-consuming and low isolation rate. Therefore, this study established a simple, rapid and effective diagnostic method to accurately diagnose and differentiate the causative fungi of dermatophytosis, which is of great significance to enhance the prevention and treatment of dermatophytosis in beef cattle farms.
Three pairs of specific primers were designed using Primer Premier 5.0 from , and . A triple PCR assay was established by optimising the primer dose and annealing temperature to improve the detection sensitivity. The feasibility of the method was verified by testing the samples.
In this study, a multiplex PCR method that can rapidly detect these three fungi at the same time was established, and its specificity, sensitivity and repeatability were analyzed at the same time. The results showed that the multiplex PCR method amplified the specific expected fragments of 581 bp, 1,513 bp and 371 bp for , and . The minimum detection limits of and were all 1 pg./μL. The positive rates were 87.5% (21/24) for samples. The results showed that the multiplex PCR method was simple, specific and sensitive and might be used for rapid diagnosis and identification of dermatophytes in cattle.
皮肤癣菌病是一种在动物和人类中常见的传染性真菌皮肤感染，是牛最常见的皮肤病。它对畜牧业有严重的负面影响。为了规避传统检测方法耗时且分离率低的缺点。因此，本研究建立了一种简单、快速且有效的诊断方法，以准确诊断和区分皮肤癣菌病的致病真菌，这对加强肉牛场皮肤癣菌病的防治具有重要意义。
使用Primer Premier 5.0软件从……设计了三对特异性引物。通过优化引物剂量和退火温度建立了三重PCR检测方法，以提高检测灵敏度。通过对样品进行检测验证了该方法的可行性。
本研究建立了一种能同时快速检测这三种真菌的多重PCR方法，并同时分析了其特异性、灵敏度和重复性。结果表明，多重PCR方法扩增出了……分别为581 bp、1513 bp和371 bp的特异性预期片段。……的最低检测限均为1 pg/μL。样品的阳性率为87.5%（21/24）。结果表明，多重PCR方法简单、特异、灵敏，可用于牛皮肤癣菌的快速诊断和鉴定。
