Al-Hammood Orooba, Muhammed Muzher Huda, Hasan Mousa Ruqaya, Vahedian Boroujeni Vala, Noory Parastoo, Mirhaj Marjan, Al-Musawi Mastafa H, Talib Al-Sudani Basma, A Mohammed Ahmed, Shahriari-Khalaji Mina, Valizadeh Hamideh, Sharifianjazi Fariborz, Bazli Leila, Tavamaishvili Ketevan, Mortazavi Moghadam Fatemeh, Tavakoli Mohamadreza
Department of Forensic Science, College of Science, Al-Nahrain University, Baghdad 10072, Iraq.
Department of Clinical Laboratory Sciences, College of Pharmacy, Mustansiriyah University, Baghdad 10052, Iraq.
ACS Appl Mater Interfaces. 2025 Apr 23;17(16):23484-23498. doi: 10.1021/acsami.4c20030. Epub 2025 Apr 8.
The skin exhibits a hierarchical structure, and the application of tissue engineering techniques is recommended for the treatment of severe cutaneous injuries. To biologically mimic the structural characteristics of the distinct layers of the skin, the utilization of multilayered scaffolds has become a prominent approach. In the current study, an asymmetric trilayered scaffold was fabricated, consisting of a middle layer (ML) composed of 3D printed poly(vinyl alcohol)-carrageenan (PVA.Crg), a top layer (TL) of nanofibrous polycaprolactone-propolis (PCL.Pp), and a bottom layer (BL) of poly(vinyl alcohol)-fucoidan-deferoxamine (PVA.Fu.Def) nanofibers. It was indicated that the tensile strength and elastic modulus of the trilayer scaffold were significantly higher compared to other samples. The degradation rate of the studied scaffolds as well as the release of Def from the trilayer scaffold after 7 days were quantified within the range of 36-40 and 91.1%, respectively. The release of Def did not induce cytotoxicity and chicken chorioallantoic membrane assay revealed that the release of Def remarkably enhanced angiogenesis. Furthermore, the examinations exhibited the fastest re-epithelialization in the group treated with the trilayer scaffold containing Def. The findings of this study suggest the potential application of the fabricated trilayer scaffold as a skin substitute or wound dressing.
皮肤呈现出分层结构，对于严重皮肤损伤的治疗，推荐应用组织工程技术。为了从生物学角度模拟皮肤不同层的结构特征，多层支架的应用已成为一种突出的方法。在当前研究中，制备了一种不对称三层支架，其由中间层（ML）、顶层（TL）和底层（BL）组成，中间层由3D打印的聚乙烯醇 - 卡拉胶（PVA.Crg）构成，顶层是纳米纤维聚己内酯 - 蜂胶（PCL.Pp），底层是聚乙烯醇 - 岩藻依聚糖 - 去铁胺（PVA.Fu.Def）纳米纤维。结果表明，与其他样品相比，三层支架的拉伸强度和弹性模量显著更高。所研究支架的降解率以及三层支架在7天后去铁胺（Def）的释放量分别在36 - 40和91.1%的范围内进行了量化。Def的释放未诱导细胞毒性，鸡胚绒毛尿囊膜试验表明Def的释放显著增强了血管生成。此外，检查显示在含有Def的三层支架治疗组中，上皮再形成最快。本研究结果表明所制备的三层支架作为皮肤替代物或伤口敷料具有潜在应用价值。
