Food Industry Research Co., Gorgan, Iran; Food and Bio-Nanotech International Research Center (Fabiano), Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Department of Mechanical and Industrial Engineering, Qatar University, Qatar.
Int J Pharm. 2023 Jul 25;642:123186. doi: 10.1016/j.ijpharm.2023.123186. Epub 2023 Jun 28.
Propolis-loaded electrospun nanofibers (PENs) have been regarded as promising candidates for biomedical purposes such as wound healing/dressing owing to their outstanding pharmacological and biological properties. This paper focuses on the development of electrospun nanofibers with optimum levels of propolis (PRP) and two polymer types (polycaprolactone (PCL) and polyvinyl alcohol (PVA)). Hence, response surface methodology (RSM) was employed to investigate the variation of the scaffold characteristics including porosity, average diameter, wettability, release, and tensile strength. For each response, a second-order polynomial model with a high coefficient of determination (R) values ranging from 0.95 to 0.989 was developed using multiple linear regression analysis. The overall optimum region with the best characteristics was found to be at PCL/6 % PRP and PVA/5 % PRP. After selecting the optimal samples, the cytotoxicity assay showed no toxicity for the optimal concentrations of PRP. Furthermore, Fourier transform infrared (FTIR) spectra revealed that no new chemical functional groups were introduced in the PENs. Uniform fibers were found in the optimum samples without the appearance of a bead-like structure in the fibers. In conclusion, nanofibers containing the optimal concentration of PRP with suitable properties can be used in biomedical and tissue engineering.
负载蜂胶的静电纺纳米纤维（PENs）由于其出色的药理和生物学特性，被认为是用于伤口愈合/敷料等生物医学目的的有前途的候选材料。本文专注于开发具有最佳蜂胶（PRP）水平和两种聚合物类型（聚己内酯（PCL）和聚乙烯醇（PVA））的静电纺纳米纤维。因此，采用响应面法（RSM）研究了支架特性的变化，包括孔隙率、平均直径、润湿性、释放和拉伸强度。对于每个响应，使用多元线性回归分析，使用高决定系数（R）值范围为 0.95 至 0.989 的二阶多项式模型进行了开发。通过整体最佳区域分析，发现具有最佳特性的区域为 PCL/6%PRP 和 PVA/5%PRP。选择最佳样品后，细胞毒性试验表明 PRP 的最佳浓度没有毒性。此外，傅里叶变换红外（FTIR）光谱表明 PENs 中没有引入新的化学官能团。在最佳样品中发现了均匀的纤维，纤维中没有出现珠状结构。总之，具有合适性能的含有最佳浓度 PRP 的纳米纤维可用于生物医学和组织工程。
