Ismaeel Farah E, Kareem Huda Raad, Abbas Marwa F, Al-Musawi Mastafa H, Kamil Marwa M, Shahriari-Khalaji Mina, Sharifianjazi Fariborz, Tavamaishvili Ketevan, Tavakoli Mohamadreza, Azamian Fariba, Mirhaj Marjan
Human Anatomy Department, College of Medicine, Mustansiriyah University, Baghdad, Iraq.
Department of Clinical Laboratory Sciences, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq.
Int J Pharm. 2025 May 15;676:125638. doi: 10.1016/j.ijpharm.2025.125638. Epub 2025 Apr 23.
Full-thickness wounds have always been one of the serious clinical problems that require a series of highly developed wound dressings to offer structural support, angiogenesis, and tissue regeneration. In the present study, an asymmetric bilayer wound dressing made up of a PVDF microfibrous top layer (TL) and a deferoxamine (Def)-loaded soluplus (Sol)-silk fibroin (SF) nanofibrous bottom layer (BL) was fabricated and characterized. After morphological and physical characterization, it was found that the presence of PVDF microfibrous layer increased tensile strength (from 3.0 ± 0.2 to 6.8 ± 0.5 MPa), elastic modulus (from 35.3 ± 3.0 to 77.9 ± 4.3 MPa), and failure strain (from 52.4 ± 2.9 to 66.8 ± 3.2 %). The swelling capability and biodegradability of the fabricated wound dressing resulted in a sustained release of Def up to approximately 88.1 ± 5.2 % in 168 h. The PVDF/Sol-SF-Def dressing showed high levels of cytocompatibility, and its superior angiogenic potential was confirmed by chicken chorioallantoic membrane assay. Histological examination demonstrated that in vivo full-thickness wounds treated with PVDF/SF-Sol-Def had superior re-epithelialization, increased granulation tissue thickness, enhanced collagen deposition, and increased COL1/COL3 ratio indicative of advanced wound remodeling. Therefore, the bilayer structure with its protective and bioactive properties represents a comprehensive approach toward the treatment of full-thickness wounds.
全层伤口一直是严重的临床问题之一，需要一系列高度发达的伤口敷料来提供结构支持、促进血管生成和组织再生。在本研究中，制备并表征了一种由聚偏氟乙烯（PVDF）微纤维顶层（TL）和负载去铁胺（Def）的索鲁普拉斯（Sol）-丝素蛋白（SF）纳米纤维底层（BL）组成的不对称双层伤口敷料。经过形态学和物理表征后发现，PVDF微纤维层的存在提高了拉伸强度（从3.0±0.2兆帕提高到6.8±0.5兆帕）、弹性模量（从35.3±3.0兆帕提高到77.9±4.3兆帕）和断裂应变（从52.4±2.9%提高到66.8±3.2%）。所制备伤口敷料的膨胀能力和生物降解性导致Def在168小时内持续释放，释放量高达约88.1±5.2%。PVDF/Sol-SF-Def敷料表现出高度的细胞相容性，其优越的血管生成潜力通过鸡胚绒毛尿囊膜试验得到证实。组织学检查表明，用PVDF/SF-Sol-Def治疗的体内全层伤口具有更好的再上皮化、增加的肉芽组织厚度、增强的胶原沉积以及增加的COL1/COL3比值，这表明伤口重塑进展良好。因此，具有保护和生物活性特性的双层结构代表了一种治疗全层伤口的综合方法。
