Flint Annika, Kumaran Dilini, Weedmark Kelly, Pagotto Franco, Ramirez-Arcos Sandra
Microbiology Research Division, Bureau of Microbial Hazards, Food and Nutrition Directorate, Health Canada, Ottawa, Ontario, Canada.
Donation Policy & Studies, Canadian Blood Services, Ottawa, Ontario, Canada.
Access Microbiol. 2025 Apr 8;7(4). doi: 10.1099/acmi.0.000938.v3. eCollection 2025.
is a bacterial skin commensal that is often isolated during routine testing of blood products like platelet concentrates (PCs). Due to the slow-growing nature of this bacterium in culture media, contaminated PCs are often transfused into vulnerable patients before retrieval of these units can be initiated. This study aimed at obtaining the whole-genome sequence of six isolates derived from contaminated PCs, comparing and assessing their genetic backgrounds. Furthermore, the whole genomes of the PC isolates were compared to clinical isolates obtained from different sites and types of infection. The results indicate that these PC isolates assessed belong to four phylotypes, namely IA, IB, II and III. Whole-genome comparisons identified differences in the virulence profiles of the isolates and provide a foundation for future studies aimed at evaluating the risk to transfusion patients by determining whether the expression of virulence factors is impacted in the PC storage environment.
是一种皮肤共生细菌，在血小板浓缩物（PCs）等血液制品的常规检测中经常分离出来。由于这种细菌在培养基中生长缓慢，受污染的PCs在能够开始回收这些单位之前，常常被输注到脆弱的患者体内。本研究旨在获得从受污染的PCs中分离出的6株菌株的全基因组序列，比较和评估它们的遗传背景。此外，还将PC分离株的全基因组与从不同部位和感染类型获得的临床分离株进行了比较。结果表明，所评估的这些PC分离株属于四个系统型，即IA、IB、II和III。全基因组比较确定了分离株毒力谱的差异，并为未来旨在通过确定毒力因子的表达在PC储存环境中是否受到影响来评估输血患者风险的研究奠定了基础。
