Buasriyot Phichaya, Supiwong Weerayuth, Muanglen Nawarat, Donbundit Nattasuda, Ditcharoen Sukhonthip, Chumpol Phonluang, Saenjundaeng Pasakorn, Tongnunui Sampan, Arunsang Sathit, Thongnetr Weera, Juntharat Sitthisak, Seetapan Kriengkrai, Liehr Thomas, Cioffi Marcelo B, Rab Petr, Tanomtong Alongklod
Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand.
Faculty of Interdisciplinary Studies, Nong Khai Campus, Khon Kaen University, Muang, Nong Khai 43000, Thailand.
Comp Cytogenet. 2025 Mar 31;19:29-50. doi: 10.3897/compcytogen.19.141557. eCollection 2025.
The barbels of the subfamilies ´Poropuntinae´ and Smiliogastrinae within the family Cyprinidae play a significant role as a food source for fish in artisanal fisheries and are highly valued as ornamental fish in Thailand. In this study, we employed both conventional and molecular cytogenetics to analyze the karyotype of 15 fish species from two cyprinid lineages. All analyzed species had a diploid chromosome number of 2n = 50. Despite sharing the same 2n, our analyses revealed species-specific distribution patterns of the mapped microsatellite motifs [(CA)₁₅, (TA)₁₅, (CAC)₁₀, and (CGG)₁₀]. They were predominantly found at telomeric sites of all-to-few chromosomes. Additionally, some species exhibited a widespread distribution of the mapped microsatellites across the chromosomes while others showed no signal. These variations reflect the evolutionary divergence and chromosomal diversity within the cyprinids. Thus, our findings support the 2n stability in cyprinoid lineages while emphasizing the intrachromosomal evolutionary diversity accompanied by species-specific microsatellite distribution.
鲤科“Poropuntinae”亚科和Smiliogastrinae亚科的触须在个体渔业中作为鱼类的食物来源发挥着重要作用，并且在泰国作为观赏鱼备受珍视。在本研究中，我们采用传统和分子细胞遗传学方法分析了来自两个鲤科谱系的15种鱼类的核型。所有分析的物种二倍体染色体数均为2n = 50。尽管共享相同的2n，但我们的分析揭示了所绘制的微卫星基序[（CA）₁₅、（TA）₁₅、（CAC）₁₀和（CGG）₁₀]的物种特异性分布模式。它们主要出现在所有到少数染色体的端粒位点。此外，一些物种在所绘制的微卫星在染色体上呈现广泛分布，而其他物种则没有信号。这些变异反映了鲤科内部的进化分歧和染色体多样性。因此，我们的研究结果支持鲤形目谱系中的2n稳定性，同时强调伴随着物种特异性微卫星分布的染色体内进化多样性。
