Khensuwan Sudarat, Supiwong Weerayuth, Suwannapoom Chatmongkon, Buasriyot Phichaya, Jantarat Sitthisak, Thongnetr Weera, Muanglen Nawarat, Kaewmad Puntivar, Saenjundaeng Pasakorn, Seetapan Kriengkrai, Liehr Thomas, Tanomtong Alongklod
Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen 40002, Thailand.
Faculty of Interdisciplinary Studies, Khon Kaen University, Nong Khai Campus, Muang, Nong Khai 43000, Thailand.
Comp Cytogenet. 2023 Sep 15;17:181-194. doi: 10.3897/compcytogen.17.107703. eCollection 2023.
Cyprininae are a highly diversified but demonstrably monophyletic lineage of cypriniform fishes. Here, the karyotype and chromosomal characteristics of (Smith, 1945) and (Smith, 1931) were examined using conventional, nucleolus organizing regions (NORs) and molecular cytogenetic protocols. The diploid chromosome number (2n) of was 50, the fundamental number (FN) was equal to 62, and the karyotype displayed 8m + 4sm + 38a with NORs located at the centromeric and telomeric positions of the short arms of chromosome pairs 1 and 2, respectively. 2n of was 50, FN 78, karyotype 14m + 14sm + 22a with the NORs at the telomeric position of the short arm of chromosome pair 2. 2n and FN in males and females were identical. Fluorescence hybridization using different microsatellite motifs as probes also showed substantial genomic divergence between both studied species. In , (CAG) and (CAC) microsatellites accumulated in the telomeric regions of all chromosomes, while in , they had scattered signals on all chromosomes. Besides, the (GAA)n microsatellites were distributed along all chromosomes of , but there was a strong hybridization pattern in the centromeric region of a single pair in . These cytogenomic difference across the genomes of these Rainboth, 1996 species are markers for specific evolutionary differentiation within these two species.
鲤亚科是鲤形目鱼类中一个高度多样化但明显为单系的谱系。在此，使用传统方法、核仁组织区（NORs）和分子细胞遗传学方案对（史密斯，1945年）和（史密斯，1931年）的核型和染色体特征进行了研究。的二倍体染色体数（2n）为50，基数（FN）等于62，核型显示为8m + 4sm + 38a，NORs分别位于第1和第2对染色体短臂的着丝粒和端粒位置。的2n为50，FN为78，核型为14m + 14sm + 22a，NORs位于第2对染色体短臂的端粒位置。雄性和雌性的2n和FN相同。使用不同微卫星基序作为探针的荧光杂交也显示，这两个研究物种之间存在显著的基因组差异。在中，（CAG）和（CAC）微卫星聚集在所有染色体的端粒区域，而在中，它们在所有染色体上有分散的信号。此外，（GAA）n微卫星分布在的所有染色体上，但在的一对染色体的着丝粒区域有强烈的杂交模式。这两个（Rainboth，1996年）物种基因组间的这些细胞基因组差异是这两个物种内特定进化分化的标志。
