Aouadi Meriem, Sebai Essia, Saratsis Anastasios, Kantzoura Vaia, Saratsi Katerina, Msaada Kamel, Sotiraki Smaragda, Akkari Hafidh
Laboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Manouba, Tunisia.
Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia.
Vet Med (Praha). 2021 Apr 2;66(4):146-155. doi: 10.17221/139/2020-VETMED. eCollection 2021 Apr.
This work aimed to evaluate the valorisation of the volatile oil of " L.", a spontaneously growing medicinal plant in Tunisia, by studying its chemical composition, anthelmintic and anticoccidial potentials against spp. and at different essential oil concentrations. The main compounds of the essential oil identified by GC/MS were three monoterpenes: 1,8-cineole (52.06%), α-pinene (15.35%) and camphor (7.69%). The anticoccidial activity was estimated by the inhibition percentage of the oocyte sporulation in addition to the unsporulated and degenerated oocysts using a haemocytometer after exposure to different essential oil concentrations. The essential oil was active against spp. oocysts of sheep at IC = 1.82 ug/ml. Therefore, the IC values of the anticoccidial activity of this oil examined was 1.82 mg/ml. The anthelmintic efficacy of the rosemary volatile oil against was realised by two tests: the egg hatch assay (EHA) and the adult worm's motility assay (AWMA), by comparing this efficacy with albendazole (anthelmintic, of reference). In the egg hatch assay, the percentage of inhibition was observed at 16 mg/ml and was 73.76% after 2 days of incubation (IC = 11.41 mg/ml) and for the adult worm's motility assay, it was 100% inhibition.
这项工作旨在通过研究突尼斯一种自然生长的药用植物“L.”挥发油的化学成分、对不同精油浓度下的 spp. 和 的驱虫及抗球虫潜力，来评估其价值。通过气相色谱/质谱法鉴定出的挥发油主要成分是三种单萜类化合物：1,8-桉叶素（52.06%）、α-蒎烯（15.35%）和樟脑（7.69%）。除了使用血细胞计数器在暴露于不同精油浓度后观察未孢子化和退化的 卵囊外，还通过卵母细胞孢子化抑制率来评估抗球虫活性。该挥发油对绵羊的 spp. 卵囊具有活性，IC = 1.82 μg/ml。因此，所检测的该油抗球虫活性的IC值为1.82 mg/ml。迷迭香挥发油对 的驱虫效果通过两项 试验实现：卵孵化试验（EHA）和成虫运动试验（AWMA），并将此效果与阿苯达唑（参考驱虫药）进行比较。在卵孵化试验中，在16 mg/ml时观察到抑制率，孵育2天后为73.76%（IC = 11.41 mg/ml），而成虫运动试验的抑制率为100%。
