Essential oil of induces anthelmintic and anticoccidial effects against and spp. in small ruminants.

This work aimed to evaluate the valorisation of the volatile oil of " L.", a spontaneously growing medicinal plant in Tunisia, by studying its chemical composition, anthelmintic and anticoccidial potentials against spp. and at different essential oil concentrations. The main compounds of the  essential oil identified by GC/MS were three monoterpenes: 1,8-cineole (52.06%), α-pinene (15.35%) and camphor (7.69%). The anticoccidial activity was estimated by the inhibition percentage of the oocyte sporulation in addition to the unsporulated and degenerated oocysts using a haemocytometer after exposure to different essential oil concentrations. The essential oil was active against spp. oocysts of sheep at IC = 1.82 ug/ml. Therefore, the IC values of the anticoccidial activity of this oil examined was 1.82 mg/ml. The anthelmintic efficacy of the rosemary volatile oil against was realised by two tests: the egg hatch assay (EHA) and the adult worm's motility assay (AWMA), by comparing this efficacy with albendazole (anthelmintic, of reference). In the egg hatch assay, the percentage of inhibition was observed at 16 mg/ml and was 73.76% after 2 days of incubation (IC = 11.41 mg/ml) and for the adult worm's motility assay, it was 100% inhibition.