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虹鳟鱼吞噬作用测定的优化（）。 （原文括号部分内容缺失，翻译可能不太完整准确）

Optimisation of phagocytosis assay in rainbow trout ().

作者信息

Minarova Hana, Ondrackova Petra, Palikova Miroslava, Mares Jan, Blahova Jana, Jarova Katerina, Faldyna Martin

机构信息

Department of Ecology & Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic.

Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic.

出版信息

Vet Med (Praha). 2021 Jun 1;66(7):298-304. doi: 10.17221/189/2020-VETMED. eCollection 2021 Jul.

DOI:10.17221/189/2020-VETMED
PMID:40201393
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11975444/
Abstract

A phagocytosis assay is one of the most commonly used functional immunological methods. There are many possible ways of assessing leucocytes and their ability to ingest different particles. The aim of this study was to optimise the phagocytosis assay in rainbow trout () using labelled zymosan particles (Alexa Fluor 488 and Texas Red conjugate). Whole blood was incubated with the particles under different conditions and leucocytes were subsequently isolated by haemolysis in a hypotonic environment. The effect of the different incubation time, temperature, blood volume and dilution on the phagocytic activity was evaluated by flow cytometry. Our experiments showed that the incubation for at least 2 h at 15 °C provided optimal results, while the blood volume and dilution had no significant effect. The optimised assay will be used for the examination of fish health and in further experimental studies.

摘要

吞噬作用测定是最常用的功能性免疫学方法之一。评估白细胞及其摄取不同颗粒的能力有多种可能的方法。本研究的目的是使用标记的酵母聚糖颗粒（Alexa Fluor 488和德克萨斯红共轭物）优化虹鳟鱼的吞噬作用测定。将全血在不同条件下与颗粒一起孵育，随后通过在低渗环境中溶血来分离白细胞。通过流式细胞术评估不同孵育时间、温度、血量和稀释度对吞噬活性的影响。我们的实验表明，在15°C下孵育至少2小时可提供最佳结果，而血量和稀释度没有显著影响。优化后的测定方法将用于鱼类健康检查和进一步的实验研究。