Jabbar Al Kaabi Hayder Kamil, Hmood Baheeja A
Basic and medical science department, College of Nursing, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq.
Open Vet J. 2025 Feb;15(2):813-819. doi: 10.5455/OVJ.2025.v15.i2.30. Epub 2025 Feb 28.
Cranberry fruit ( L) contains a mixture of phytochemicals such as flavonoids which consist of flavonoids, anthocyanins, proanthocyanidins, catechins, phenolic acids, and triterpenoids, all of which have possible antimicrobial (antibacterial, antifungal, and antiviral) activity.
This study aimed to determine the antibacterial effect of the ethanol extract of cranberry juice (CJ) against uropathogen commonly found in urinary tract infections (UTIs) associated with pregnancy.
Cranberry fruits were purchased from local markets and juiced, filtered, and dried. The dried powder was extracted with 70% ethanol for 8-10 days. The bacterial isolates used in this study [, , , sp., and coagulase-negative (CNS)] were collected from the Maternity and Children Hospital in Al-Diwaniyah City, Iraq. The antibacterial activity of the ethanol extract of CJ was measured using a standard Disc diffusion method. Sterile paper discs were soaked in 20 µl of different concentrations (12.5, 25, 50, and 100 mg/ml) of the extract, placed in Mueller-Hinton agar plates, and inoculated with bacterial cultures adjusted to 0.5 McFarland standards. Amoxicillin (30 µg) was used as a positive control, and 70% ethanol was used as a negative control.
The ethanol solution of CJ displayed a significant ( < 0.05) inhibitory activity against all tested bacteria. The CNS showed the highest sensitivity with 100% inhibition, followed by (90%), sp. (85%), (75%), and (60%). The effect was dose dependent, as increasing the extract concentration resulted in broader inhibition zones.
The results show that the ethanol extract of CJ has potent antibacterial activity against uropathogenic bacteria, suggesting a possible role for CJ ethanol extract in treating UTIs when combined with recently emerging facts about increasing antibiotic resistance.
蔓越莓果实（L）含有多种植物化学物质的混合物，如黄酮类化合物，其中包括黄酮、花青素、原花青素、儿茶素、酚酸和三萜类化合物，所有这些都可能具有抗菌（抗菌、抗真菌和抗病毒）活性。
本研究旨在确定蔓越莓汁（CJ）乙醇提取物对与妊娠相关的尿路感染（UTIs）中常见的尿路病原体的抗菌作用。
从当地市场购买蔓越莓果实，榨汁、过滤并干燥。将干燥粉末用70%乙醇提取8 - 10天。本研究中使用的细菌分离株[、、、sp.和凝固酶阴性（CNS）]从伊拉克迪瓦尼亚市的妇幼医院收集。使用标准纸片扩散法测量CJ乙醇提取物的抗菌活性。将无菌纸片浸泡在20μl不同浓度（12.5、25、50和100mg/ml）的提取物中，置于穆勒-欣顿琼脂平板上，并用调整至0.5麦氏标准的细菌培养物接种。阿莫西林（30μg）用作阳性对照，70%乙醇用作阴性对照。
CJ的乙醇溶液对所有测试细菌均表现出显著（<0.05）的抑制活性。CNS显示出最高的敏感性，抑制率为100%，其次是（90%）、sp.（85%）、（75%）和（60%）。这种作用呈剂量依赖性，因为提取物浓度增加会导致更宽的抑菌圈。
结果表明，CJ乙醇提取物对尿路致病细菌具有强大的抗菌活性，这表明在抗生素耐药性不断增加的新情况下，CJ乙醇提取物在治疗UTIs中可能发挥作用。
