Li Ke, Song Jia, Lu Yanhui, Zhang Daixing, Wang Yuqing, Wang Xinyu, Tang Yujing, Yu Yingjie, Zhang Xuehui, Yang Xiaoping, Cai Qing
State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
SINOPEC (Beijing) Research Institute of Chemical Industry Co., Ltd., Beijing 100013, China.
ACS Appl Mater Interfaces. 2025 Apr 23;17(16):23707-23721. doi: 10.1021/acsami.5c02557. Epub 2025 Apr 9.
Guided tissue regeneration (GTR) using barrier membranes is a common clinical approach for treating periodontitis-induced alveolar bone loss. However, conventional GTR membranes lack antibacterial and osteoinductive properties, limiting their effectiveness. Piezoelectric materials, which generate electrical outputs under chewing forces, offer antibacterial and bone-regenerative potential due to their oppositely charged surfaces. Inspired by this, a piezoelectric Janus membrane was developed for dual-function GTR therapy. Biodegradable poly(l-lactide) (PLLA) and PLLA/gelatin membranes were electrospun, annealed, and polarized to create the A-P(+)/PG(-) piezoelectric Janus membrane. Notably, in this Janus membrane, the outer surface of the PLLA side (A-P(+)) carries positive charges and is positioned toward the gingival tissue to kill bacteria via charge interactions; the inner surface of the PG side (PG(-)) holds negative charges and faces the alveolar bone defect, promoting bone growth through immunomodulation and enhanced mineralization. In a mouse model of periodontitis, the Janus membrane A-P(+)/PG(-) demonstrated dual functionality, effectively reducing inflammation, inhibiting bone resorption. The bone mineral density of A-P(+)/PG(-) reached 1637 ± 37 mg/cm at 8 weeks after surgery, which was superior to commercial collagen membranes lacking antibacterial properties. Overall, this study introduces an innovative approach, leveraging biodegradable piezoelectric PLLA to construct a versatile Janus GTR membrane with enhanced antibacterial and osteogenic activity for periodontitis treatment.
使用屏障膜的引导组织再生（GTR）是治疗牙周炎引起的牙槽骨丧失的常见临床方法。然而，传统的GTR膜缺乏抗菌和骨诱导特性，限制了它们的有效性。压电材料在咀嚼力作用下会产生电输出，由于其带相反电荷的表面而具有抗菌和骨再生潜力。受此启发，开发了一种用于双重功能GTR治疗的压电Janus膜。对可生物降解的聚（L-丙交酯）（PLLA）和PLLA/明胶膜进行静电纺丝、退火和极化处理，以制备A-P(+)/PG(-)压电Janus膜。值得注意的是，在这种Janus膜中，PLLA侧（A-P(+)）的外表面带正电荷，朝向牙龈组织，通过电荷相互作用杀死细菌；PG侧（PG(-)）的内表面带负电荷，面对牙槽骨缺损，通过免疫调节和增强矿化促进骨生长。在牙周炎小鼠模型中，Janus膜A-P(+)/PG(-)显示出双重功能，有效减轻炎症，抑制骨吸收。术后8周，A-P(+)/PG(-)的骨矿物质密度达到1637±37mg/cm，优于缺乏抗菌性能的商业胶原膜。总体而言，本研究引入了一种创新方法，利用可生物降解的压电PLLA构建一种多功能Janus GTR膜，具有增强的抗菌和成骨活性，用于治疗牙周炎。
