Huang Lin, Wu Tao, Sun Jing, Lin Xinghuan, Peng Yuhao, Zhang Rongrong, Gao Yang, Xu Shuo, Sun Yuxin, Zhou Yi, Duan Bo
Interdisciplinary Institute of NMR and Molecular Sciences, School of Chemistry and Chemical Engineering, The State Key Laboratory of Refractories and Metallurgy, Wuhan University of Science and Technology, Wuhan 430081, China; College of Chemistry and Molecular Sciences, Hubei Engineering Center of Natural Polymer-based Medical Materials, Wuhan University, Wuhan 430072, China.
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Acta Biomater. 2024 Dec;190:219-232. doi: 10.1016/j.actbio.2024.10.038. Epub 2024 Oct 24.
Periodontal defects caused by severe periodontitis are a widespread issue globally. Guided tissue regeneration (GTR) using barrier membranes for alveolar bone repair is a common clinical treatment. However, most commercially available collagen barrier membranes are expensive and lack the antibacterial properties essential for effective bone regeneration. Herein, we report a natural polysaccharide chitin hydrogel barrier membrane with a Janus structure (ChT-PDA-p-HAP), featuring high antibacterial and protein-repelling activity on the outer side and good osteogenesis ability on the inner side. This multifunctional membrane is fabricated though a three-step process: (i) dissolution and regeneration of chitin, (ii) co-deposition with polydopamine (PDA) and poly(sulfobetaine methacrylate) (pSBMA), and (iii) coating with gelatin-hydroxyapatite (gelatin-HAP). In vitro cell experiments demonstrated the membrane's high biocompatibility and significant osteogenic activity. In vivo implantation in rats with periodontal defects revealed that the cemento-enamel junction index of the ChT-PDA-p-HAP membrane (1.165 mm) was superior to that of the commercial Bio-Gide® membrane (1.350 mm). This work presents a method for fabricating a chitin-based Janus barrier membrane, potentially expanding the use of chitin in tissue engineering. STATEMENT OF SIGNIFICANCE: This study introduces a Janus hydrogel membrane based on chitin, tailored for guided tissue regeneration in periodontal defects. By combining antibacterial properties and osteogenic capabilities in a single membrane, the ChT-PDA-p-HAP membrane represents a significant advancement over traditional collagen barriers. Its outer surface, enhanced by Cu and PDA-pSBMA coatings, resists bacterial colonization and protein adhesion effectively, while the inner side, coated with gelatin-HAP, promotes robust bone formation. In vitro experiments demonstrate high biocompatibility and substantial osteogenic differentiation, while in vivo testing in rat models confirms good therapeutic efficacy compared to commercial membranes. This multifunctional approach not only utilizes chitin's abundant natural resource but also integrates simple coating techniques to enhance therapeutic outcomes in periodontal tissue engineering, offering promising avenues for broader biomedical applications.
重度牙周炎导致的牙周缺损是全球范围内普遍存在的问题。使用屏障膜进行引导组织再生（GTR）以修复牙槽骨是一种常见的临床治疗方法。然而，大多数市售的胶原屏障膜价格昂贵，且缺乏有效骨再生所必需的抗菌性能。在此，我们报道了一种具有Janus结构的天然多糖几丁质水凝胶屏障膜（ChT-PDA-p-HAP），其外侧具有高抗菌和抗蛋白活性，内侧具有良好的成骨能力。这种多功能膜通过三步工艺制备：（i）几丁质的溶解和再生，（ii）与聚多巴胺（PDA）和聚（甲基丙烯酰基磺酸甜菜碱）（pSBMA）共沉积，以及（iii）用明胶-羟基磷灰石（明胶-HAP）涂层。体外细胞实验证明了该膜具有高生物相容性和显著的成骨活性。在患有牙周缺损的大鼠体内植入实验表明，ChT-PDA-p-HAP膜的牙骨质-釉质界指数（1.165毫米）优于市售的Bio-Gide®膜（1.350毫米）。这项工作提出了一种制备基于几丁质的Janus屏障膜的方法，有可能扩大几丁质在组织工程中的应用。意义声明：本研究引入了一种基于几丁质的Janus水凝胶膜，专为牙周缺损的引导组织再生而设计。通过在单个膜中结合抗菌性能和成骨能力，ChT-PDA-p-HAP膜代表了相对于传统胶原屏障的重大进步。其外表面通过铜和PDA-pSBMA涂层得到增强，有效地抵抗细菌定植和蛋白质粘附，而内侧涂有明胶-HAP，促进强大的骨形成。体外实验证明了高生物相容性和大量的成骨分化，而在大鼠模型中的体内测试证实与市售膜相比具有良好的治疗效果。这种多功能方法不仅利用了几丁质丰富的天然资源，还整合了简单的涂层技术以提高牙周组织工程中的治疗效果，为更广泛的生物医学应用提供了有希望的途径。
