Cheng Jingrong, Wu Liping, Fu Hongying, Hu Le, Wang Weijia, Heng Boon Chin, Zhang Xuehui, Liu Ousheng, Deng Xuliang, Liu Yang
Xiangya Stomatological Hospital and Xiangya School of Stomatology, Central South University & Hunan Engineering Research Center for Oral Digital Intelligence and Personalized Medicine, 72 Xiangya Road, Changsha, Hunan, 410008, P. R. China.
Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing, 100081, P. R. China.
Adv Healthc Mater. 2025 May;14(12):e2500543. doi: 10.1002/adhm.202500543. Epub 2025 Mar 26.
An ideal guided bone regeneration (GBR) membrane for periodontitis treatment should incorporate biocompatibility, biodegradability, mechanical strength, antibacterial properties, and osteoconductivity. However, no commercially available GBR membrane meets all these criteria simultaneously. In this study, a novel biodegradable piezoelectric double-layered membrane is developed, with a non-piezoelectric Poly-L-lactic acid (PLLA) side facing the gingiva and a piezoelectric PLLA-ZnO side facing the alveolar bone. This asymmetric GBR membrane, with distinct fiber orientations and charge distribution, combines and synergizes mechanical strength, degradability, barrier function, antibacterial activity and osteogenic potential to enhance bone regeneration efficacy. The GBR membrane can effectively prevent fibroblast migration, inhibits bacterial infection, and promotes bone regeneration both in vitro and in vivo. In vitro testing shows good antibacterial rate against Porphyromonas gingivalis (P. gingivalis) and Staphylococcus aureus (S. aureus) after 10 min of ultrasound stimulation. Expression levels of osteogenic genes Bone morphogenetic Protein 2 (BMP2), Runt-related transcription factor 2 (RUNX2), Osteopontin (OPN) and Osteocalcin (OCN) are over twice that of the control. In a mouse P. gingivalis-mediated periodontitis model, our composite membrane demonstrates effective antimicrobial effects and promote bone regeneration after 2- and 4-weeks implantation, facilitated by mechanisms such as physical isolation, zinc ion release, piezoelectric effects, enhanced expression of osteogenic genes through activation of osteogenesis-related signaling pathways, underscoring its strong potential for GBR applications.
一种用于牙周炎治疗的理想引导骨再生（GBR）膜应具备生物相容性、生物可降解性、机械强度、抗菌性能和骨传导性。然而，目前尚无市售的GBR膜能同时满足所有这些标准。在本研究中，开发了一种新型的可生物降解压电双层膜，其面向牙龈的一侧为非压电聚-L-乳酸（PLLA），面向牙槽骨的一侧为压电PLLA-ZnO。这种不对称的GBR膜具有独特的纤维取向和电荷分布，结合并协同了机械强度、降解性、屏障功能、抗菌活性和成骨潜力，以提高骨再生效果。该GBR膜能有效防止成纤维细胞迁移，抑制细菌感染，并在体外和体内促进骨再生。体外测试表明，在超声刺激10分钟后，对牙龈卟啉单胞菌（P. gingivalis）和金黄色葡萄球菌（S. aureus）具有良好的抗菌率。成骨基因骨形态发生蛋白2（BMP2）、 runt相关转录因子2（RUNX2）、骨桥蛋白（OPN）和骨钙素（OCN）的表达水平是对照组的两倍以上。在小鼠牙龈卟啉单胞菌介导的牙周炎模型中，我们的复合膜在植入2周和4周后显示出有效的抗菌作用并促进骨再生，其机制包括物理隔离、锌离子释放、压电效应、通过激活成骨相关信号通路增强成骨基因的表达，突出了其在GBR应用中的强大潜力。
