Chen Jiamin, Wang Xingran, Li Xin, Li Xiaoli, Zhang Yiyao, Yuan Yi
School of Electrical Engineering, Yanshan University, Qinhuangdao, China.
Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province, Yanshan University, Qinhuangdao, China.
CNS Neurosci Ther. 2025 Apr;31(4):e70351. doi: 10.1111/cns.70351.
Neurological diseases like Alzheimer's disease (AD) with cognitive deficits show impaired theta, gamma, and ripple bands. Restoring these oscillations may be crucial for rescuing cognitive functions. Low-intensity transcranial ultrasound stimulation (TUS), a noninvasive neuromodulation method, offers high spatial resolution and deep penetration. However, it remains unclear how 40 Hz and 200 Hz TUS may improve memory in AD by regulating hippocampal oscillations.
We applied 40 Hz and 200 Hz TUS to the CA1 region of AD mice, performing memory assessments and CA1 electrophysiology recordings simultaneously.
Our results showed that both 40 Hz and 200 Hz TUS significantly improved memory performance in AD mice by targeting the dorsal hippocampus and increasing power in corresponding frequency bands. Specifically, 40 Hz TUS enhanced gamma and ripple bands, while 200 Hz TUS strongly affected both. This enhancement increased during stimulation and persisted 5 days poststimulation. Improved coupling between theta and gamma oscillations indicated better hippocampal coordination with other brain regions. Additionally, 40 Hz TUS raised sharp wave ripple (SPW-Rs) incidence, and 200 Hz TUS increased both SPW-R incidence and duration, contributing to memory improvement. Behavioral performance significantly improved with TUS at both frequencies.
Ultrasound-induced synchronized neural activities at 40 Hz and 200 Hz entrained corresponding oscillations and improved memory in Alzheimer's disease.
像阿尔茨海默病（AD）这种伴有认知缺陷的神经疾病，其θ波、γ波和涟漪波频段存在功能受损。恢复这些振荡可能对挽救认知功能至关重要。低强度经颅超声刺激（TUS）是一种非侵入性神经调节方法，具有高空间分辨率和深部穿透性。然而，40赫兹和200赫兹的TUS如何通过调节海马振荡来改善AD患者的记忆仍不清楚。
我们将40赫兹和200赫兹的TUS应用于AD小鼠的CA1区，同时进行记忆评估和CA1区电生理记录。
我们的结果表明，40赫兹和200赫兹的TUS均通过靶向背侧海马体并增加相应频段的功率，显著改善了AD小鼠的记忆表现。具体而言，40赫兹的TUS增强了γ波和涟漪波频段，而200赫兹的TUS对两者均有强烈影响。这种增强在刺激期间增加，并在刺激后持续5天。θ波和γ波振荡之间更好的耦合表明海马体与其他脑区的协调性更好。此外，40赫兹的TUS提高了尖波涟漪（SPW-Rs）的发生率，200赫兹的TUS增加了SPW-R的发生率和持续时间，有助于记忆改善。两种频率的TUS均使行为表现显著改善。
40赫兹和200赫兹的超声诱导同步神经活动带动了相应振荡，改善了阿尔茨海默病患者的记忆。
