Dorado Jesús, Ortiz Isabel
Department of Animal Medicine and Surgery, University of Córdoba, Córdoba, Spain.
Methods Mol Biol. 2025;2897:147-157. doi: 10.1007/978-1-0716-4406-5_10.
Cryopreservation of dog semen is an assisted reproductive technology of the utmost importance for increasing the genetic pool and developing the dog breeding industry. This technique is not unharmful for the sperm, and factors such as extender, cryoprotectant, and cooling-warming rates have been shown to affect the after-thawing quality of canine sperm.This chapter aims to describe the current range of methodologies available that are key to ensure successful freezing and thawing of dog sperm.
犬精液冷冻保存是一项极为重要的辅助生殖技术，对于扩大基因库和发展犬类繁育产业具有重要意义。该技术对精子并非毫无损害，已有研究表明，稀释液、冷冻保护剂以及降温-复温速率等因素会影响犬精子的解冻后质量。本章旨在描述当前可用的一系列关键方法，这些方法对于确保犬精子的成功冷冻和解冻至关重要。
