Wang Yueru, Wang Zhichao, Pan Jiaojiao, Wang He, Lei Ziwen, Liu Jing, Zou Junbo, Lv Haizhen, Luan Fei
College of Medicine and Pharmacy, Shaanxi Institute of International Trade & Commerce, Xi'an, Shaanxi, P.R. China.
Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, School of Pharmacy, Shaanxi University of Chinese Medicine, Xi'an, Shaanxi, P.R. China.
PLoS One. 2025 Apr 9;20(4):e0320855. doi: 10.1371/journal.pone.0320855. eCollection 2025.
Long non-coding RNAs (lncRNAs) exert their pharmacological functions by serving as sponges for related microRNAs (miRNAs), thereby modulating gene expression. Nevertheless, the regulatory roles of the lncRNA-mediated competing endogenous RNA (ceRNA) mechanism in the interplay between burns and keloids remain largely elusive.
To construct the ceRNA regulatory network of burns, leveraging network pharmacology and bioinformatics analyses.
3576 DELs (Differentially Expressed lncRNAs), 1427 DEMis (Differentially Expressed miRNAs), and 2555 DEMs (Differentially Expressed mRNAs) were identified as differentially expressed genes. A ceRNA network composed of DELs-DEMis-DEMs in burns and keloids was constructed, with a prominent sub-network consisting of 23 DELs, 330 DEMs, and 8 DEMis. Subsequently, the clusterProfiler package in the R programming language was utilized to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The sub-network within the ceRNA network was extracted, in which three lncRNAs, namely lnc-WRB, lnc-SCNN1G, and LINC00271, and three miRNAs, namely hsa-miR-21, hsa-miR-34a, and hsa-miR-155, were identified as key genes.
All nodes within the sub-ceRNA network exert either a direct or an indirect influence on the pathological processes of burns and post-burn keloids. The current study successfully constructed the ceRNA network in burns and keloids and provided a potentially novel perspective on the DELs-DEMis-DEMs ceRNA network, contributing to the elucidation of the ceRNA regulatory mechanisms in the pathogenesis of burns and keloids. Nevertheless, systematic and rigorous experimental validations are indispensable to confirm our findings.
长链非编码RNA（lncRNAs）通过充当相关微小RNA（miRNAs）的海绵来发挥其药理功能，从而调节基因表达。然而，lncRNA介导的竞争性内源RNA（ceRNA）机制在烧伤与瘢痕疙瘩相互作用中的调控作用仍 largely难以捉摸。
利用网络药理学和生物信息学分析构建烧伤的ceRNA调控网络。
3576个差异表达lncRNA（DELs）、1427个差异表达miRNA（DEMis）和2555个差异表达mRNA（DEMs）被鉴定为差异表达基因。构建了烧伤和瘢痕疙瘩中由DELs-DEMis-DEMs组成的ceRNA网络，其中一个由23个DELs、330个DEMs和8个DEMis组成的显著子网。随后，利用R编程语言中的clusterProfiler包进行基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析。提取ceRNA网络中的子网，其中三个lncRNA，即lnc-WRB、lnc-SCNN1G和LINC00271，以及三个miRNA，即hsa-miR-21、hsa-miR-34a和hsa-miR-155，被鉴定为关键基因。
ceRNA子网内的所有节点对烧伤和烧伤后瘢痕疙瘩的病理过程均有直接或间接影响。本研究成功构建了烧伤和瘢痕疙瘩中的ceRNA网络，并为DELs-DEMis-DEMs ceRNA网络提供了一个潜在的新视角，有助于阐明烧伤和瘢痕疙瘩发病机制中的ceRNA调控机制。然而，需要系统而严格的实验验证来证实我们的发现。
