在英约小单孢菌中鉴定出以前在放线菌中未发现的氨基糖苷乙酰转移酶活性。
Characterization in Micromonospora inyoensis of aminoglycoside acetyltransferase activity not previously encountered among actinomycetes.
作者信息
Salas J A, Cundliffe E
出版信息
J Gen Microbiol. 1985 Mar;131(3):451-7. doi: 10.1099/00221287-131-3-451.
Extracts of Micromonospora inyoensis contain aminoglycoside acetyltransferase activity not previously encountered among actinomycetes. Neomycin and its derivatives (excluding butirosin) are good substrates as is the novel aminoglycoside apramycin, whereas the gentamicins and kanamycins are utilized poorly if at all. Sisomicin, which is produced by M. inyoensis, is not acetylated. When neomycin was modified by extracts of M. inyoensis the product, most probably 3-N-acetylneomycin, was inactive against cell-free protein synthesis.
英约小单孢菌提取物含有氨基糖苷乙酰转移酶活性，这在放线菌中是以前未曾遇到过的。新霉素及其衍生物（不包括布替罗星）以及新型氨基糖苷阿泊拉霉素都是良好的底物，而庆大霉素和卡那霉素即使被利用，利用率也很低。由英约小单孢菌产生的西索米星不会被乙酰化。当新霉素被英约小单孢菌提取物修饰时，产物很可能是3-N-乙酰新霉素，它对无细胞蛋白质合成没有活性。
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