Hedges R W, Shannon K P
J Gen Microbiol. 1984 Mar;130(3):473-82. doi: 10.1099/00221287-130-3-473.
The mechanisms of resistance to apramycin of five isolates of Escherichia coli from animals were investigated. Three isolates, which were resistant to all the aminoglycosides tested, did not transfer their resistance and did not produce aminoglycoside-modifying enzymes. The fourth isolate, which was resistant to apramycin, tobramycin, gentamicin, kanamycin and neomycin but not to amikacin, owed its resistance to production of the acetyltransferase AAC(3)IV. The gene specifying this enzyme was carried on a transposon, Tn800, on a plasmid designated R1535. The fifth isolate was resistant to apramycin, neomycin and kanamycin but not to gentamicin, tobramycin or amikacin. It produced an acetyltransferase that readily acetylated only apramycin, neomycin and paromomycin, a compound that is closely related to neomycin. Synthesis of this enzyme was specified by a chromosomal gene located near pyrD at about 20 min on the map of the E. coli K12 chromosome.
对五株动物源大肠杆菌对阿普拉霉素的耐药机制进行了研究。三株对所有测试氨基糖苷类药物均耐药的菌株，其耐药性既不转移，也不产生氨基糖苷类修饰酶。第四株对阿普拉霉素、妥布霉素、庆大霉素、卡那霉素和新霉素耐药，但对阿米卡星不耐药，其耐药性归因于乙酰转移酶AAC(3)IV的产生。编码该酶的基因位于转座子Tn800上，该转座子位于名为R1535的质粒上。第五株对阿普拉霉素、新霉素和卡那霉素耐药，但对庆大霉素、妥布霉素或阿米卡星不耐药。它产生一种乙酰转移酶，该酶仅能轻易地使阿普拉霉素、新霉素和巴龙霉素（一种与新霉素密切相关的化合物）乙酰化。这种酶的合成由位于大肠杆菌K12染色体图谱上约20分钟处pyrD附近的一个染色体基因所决定。
