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由庆大霉素产生菌紫色小单孢菌的DNA所确定的16S核糖体RNA甲基化以及对氨基糖苷类抗生素庆大霉素和卡那霉素的抗性。

Methylation of 16S ribosomal RNA and resistance to the aminoglycoside antibiotics gentamicin and kanamycin determined by DNA from the gentamicin-producer, Micromonospora purpurea.

作者信息

Thompson J, Skeggs P A, Cundliffe E

出版信息

Mol Gen Genet. 1985;201(2):168-73. doi: 10.1007/BF00425655.

DOI:10.1007/BF00425655
PMID:3866905
Abstract

When DNA fragments from Micromonospora purpurea (the producer of gentamicin) were cloned in Streptomyces lividans, a gentamicin-resistant strain was obtained in which the ribosomes were highly resistant both to gentamicin and to kanamycin. Reconstitution analysis revealed that such resistance resulted from some property of their 16S RNA. Extracts from the clone contained methylase activity which acted on 16S RNA within E. coli 30S ribosomal subunits and rendered them resistant to gentamicin and kanamycin.

摘要

当来自紫色小单孢菌（庆大霉素的产生菌）的DNA片段克隆到变铅青链霉菌中时，获得了一个对庆大霉素耐药的菌株，该菌株的核糖体对庆大霉素和卡那霉素都具有高度抗性。重组分析表明，这种抗性是由其16S RNA的某些特性导致的。该克隆的提取物含有甲基化酶活性，该活性作用于大肠杆菌30S核糖体亚基内的16S RNA，使其对庆大霉素和卡那霉素产生抗性。

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