Ding Xiaoqin, Zhao Yabin, Zheng Renlin, Cai Jimeng, Peng Xinyu, Li Liangchun
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
Int J Biol Macromol. 2025 May;309(Pt 2):142938. doi: 10.1016/j.ijbiomac.2025.142938. Epub 2025 Apr 7.
In this study, we developed an ultrasound-assisted dual-enzyme hydrolysis method for extracting low-molecular-weight peptides from maize alcohol-soluble proteins. Using a Box-Behnken design within response surface methodology (RSM), we optimized the hydrolysis process, achieving a maximum protein hydrolysis degree of 26.5 % under optimal conditions: substrate concentration 4 g/L, pH 8.3, enzyme addition 6 %, ultrasonic power 480 W/L, ultrasonic duration 2.3 h, and hydrolysis temperature 60 °C. Structural analysis revealed that ultrasound-treated samples exhibited looser structures and more wrinkled surfaces, indicative of increased exposure of hydrophobic and aromatic amino acids. In vitro antioxidant assays demonstrated significantly higher DPPH, hydroxyl radical, and superoxide anion radical scavenging activities in ultrasound-treated samples (p < 0.05), with the <3 kDa peptide fraction showing the highest activity. This study highlights the potential of ultrasound-assisted dual-enzyme hydrolysis for enhancing zein utilization and advancing sustainable food additive development.
在本研究中，我们开发了一种超声辅助双酶水解法，用于从玉米醇溶蛋白中提取低分子量肽。采用响应面法（RSM）中的Box-Behnken设计，我们优化了水解过程，在最佳条件下实现了最大蛋白质水解度26.5%：底物浓度4 g/L、pH 8.3、酶添加量6%、超声功率480 W/L、超声持续时间2.3 h以及水解温度60℃。结构分析表明，经超声处理的样品结构更松散，表面更皱，表明疏水和芳香族氨基酸的暴露增加。体外抗氧化试验表明，经超声处理的样品对DPPH、羟基自由基和超氧阴离子自由基的清除活性显著更高（p < 0.05），<3 kDa肽段的活性最高。本研究突出了超声辅助双酶水解在提高玉米醇溶蛋白利用率和推动可持续食品添加剂开发方面的潜力。
