College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China; Department of Animal Science, Faculty of Agriculture, University of Benin City, Nigeria.
Ultrason Sonochem. 2024 Aug;108:106944. doi: 10.1016/j.ultsonch.2024.106944. Epub 2024 Jun 6.
With the proposal of the 2030 Agenda for Sustainable Development, the Chinese medicine extraction technology has been innovatively improved to prioritize low energy consumption, sustainability, and minimized organic solvent utilization. Forsythia suspensa (FS) possesses favorable pharmacological properties and is extensively utilized in traditional Chinese medicine. However, due to the limitations of the composition and extraction methods, its potential has not been fully developed. Thus, a combination of ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), and β-cyclodextrin extraction (β-CDE) was employed to isolate and purify rutin, phillyrin, and forsythoside A from FS. The results demonstrated that the efficiency of extracting enzymatic and ultrasound assisted β-cyclodextrin extraction (EUA-β-CDE) was highly influenced by the temperature and duration of hydrolysis, as well as the duration of the extraction process. According to the results of the single-factor experiment, Box-Behnken design (BBD) in Response surface method (RSM) was used to optimize the experimental parameters to achieve the maximum comprehensive evaluation value (CEV) value. The EUA-β-CDE compared with other extraction methods, has good extraction effect and low energy consumption by high performance liquid chromatography (HPLC), scanning electron microscopy (SEM), calculation of power consumption and CO emission The EUA-β-CDE compared with other extraction methods, has good extraction effect and low energy consumption by HPLC, SEM, calculation of power consumption and CO emission. Then, the structural characteristics of EUA-β-CDE of FS extract had significant interaction with β-CD by Fourier infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC). In addition, EUA-β-CDE extract has good antioxidant and anti-inflammatory activities. The establishment of EUA-β-CDE of FS provides a new idea for the development and application of other sustainable extraction methods of traditional Chinese medicine.
随着 2030 年可持续发展议程的提出，中药提取技术创新性地得到了改进，以优先考虑低能耗、可持续性和最小化有机溶剂的使用。连翘（FS）具有良好的药理性质，广泛应用于中药。然而，由于成分和提取方法的限制，其潜力尚未得到充分开发。因此，采用超声辅助提取（UAE）、酶辅助提取（EAE）和β-环糊精提取（β-CDE）相结合的方法，从 FS 中分离和纯化芦丁、连翘苷和连翘酯苷 A。结果表明，酶解和超声辅助β-环糊精提取（EUA-β-CDE）的效率受到水解温度和时间以及提取过程时间的强烈影响。根据单因素实验结果，采用响应面法（RSM）中的 Box-Behnken 设计（BBD）对实验参数进行优化，以获得最大综合评价值（CEV）值。与其他提取方法相比，EUA-β-CDE 具有良好的提取效果和低能耗，通过高效液相色谱法（HPLC）、扫描电子显微镜（SEM）、功耗和 CO 排放量的计算进行了验证。与其他提取方法相比，EUA-β-CDE 具有良好的提取效果和低能耗，通过高效液相色谱法（HPLC）、扫描电子显微镜（SEM）、功耗和 CO 排放量的计算进行了验证。然后，通过傅里叶变换红外光谱（FT-IR）和差示扫描量热法（DSC）对 FS 提取物的 EUA-β-CDE 的结构特征与β-CD 进行了分析，发现其具有显著的相互作用。此外，EUA-β-CDE 提取物具有良好的抗氧化和抗炎活性。FS 的 EUA-β-CDE 的建立为其他可持续中药提取方法的开发和应用提供了新的思路。
