Beske O, Gorenstein C
LBVAMC, Long Beach, CA 90822.
Brain Res. 1995 Jan 9;669(1):125-9. doi: 10.1016/0006-8993(94)01201-r.
Lysosomes in hypoglossal motoneurons were retrogradely labeled with fluorescent latex microspheres and their distribution as well as that of acid phosphatase was examined after a single 50 microgram intracerebroventricular (i.c.v.) injection of colchicine. In saline injected controls the fluorescent label was distributed mainly in cell bodies. Twenty-four hours after the colchicine injection we observed a re-distribution of fluorescent label from the cell body of neurons to the dendrites. Seventy-two hours after the colchicine injection the fluorescent label had returned from the dendrites to the cell body. A similar pattern was obtained by following the effect of colchicine on the distribution of acid phosphatase reaction product. We conclude that the reappearance of fluorescent label in cell bodies following colchicine treatment is the result of the retrograde transport of dendritic lysosomes.
用荧光乳胶微球对舌下运动神经元中的溶酶体进行逆行标记，并在脑室内单次注射50微克秋水仙碱后，检查其分布以及酸性磷酸酶的分布。在注射生理盐水的对照组中，荧光标记主要分布在细胞体中。注射秋水仙碱24小时后，我们观察到荧光标记从神经元的细胞体重新分布到树突。注射秋水仙碱72小时后，荧光标记从树突回到细胞体。通过追踪秋水仙碱对酸性磷酸酶反应产物分布的影响，也得到了类似的模式。我们得出结论，秋水仙碱处理后细胞体中荧光标记的重新出现是树突状溶酶体逆行运输的结果。
