Kamimura H, Sasaki H, Yokoi K, Kawamura S
J Pharm Sci. 1985 May;74(5):559-61. doi: 10.1002/jps.2600740514.
Two methods have been developed for the quantitative determination of indeloxazine, a cerebral activator, in plasma by HPLC or GC-MS. After addition of viloxazine as the internal standard, indeloxazine was extracted from alkalinized plasma. In the HPLC method, the plasma extract was treated with 5-dimethylamino-1-naphthalenesulfonyl chloride and analyzed by HPLC with fluorescence detection. In the GC-MS method, the two plasma compounds were converted to their pentafluoropropionyl derivatives for selected-ion monitoring. The limits of detection were 5 and 2 ng/mL for the HPLC and GC-MS methods, respectively. When plasma samples obtained by giving indeloxazine hydrochloride to volunteers were analyzed by the two methods, good correlation between the data was obtained (r = 0.9901).
已开发出两种通过高效液相色谱法（HPLC）或气相色谱-质谱联用（GC-MS）对血浆中脑激活剂茚达雷司进行定量测定的方法。加入维拉佐酮作为内标后，从碱化血浆中提取茚达雷司。在HPLC法中，血浆提取物用5-二甲基氨基-1-萘磺酰氯处理，并用带荧光检测的HPLC进行分析。在GC-MS法中，将这两种血浆化合物转化为它们的五氟丙酰基衍生物用于选择离子监测。HPLC法和GC-MS法的检测限分别为5 ng/mL和2 ng/mL。当用这两种方法分析给志愿者服用盐酸茚达雷司后获得的血浆样品时，数据之间具有良好的相关性（r = 0.9901）。
