Kleeman W P, Bailey L C
J Pharm Sci. 1985 Jun;74(6):655-9. doi: 10.1002/jps.2600740615.
Two independent, simplex procedures were employed to optimize the official blue tetrazolium reaction. The net absorbance for a fixed amount of hydrocortisone was maximized by the simultaneous variation of five factors of the pharmacopeial method, i.e.: concentrations of tetramethylammonium hydroxide, blue tetrazolium, and water, as well as temperature and time for color development. An initial simplex was used to determine an optimum response, while a second was performed to minimize the blank absorbance obtained at the optimum. A series of pharmaceutically important steroids: prednisone, prednisolone, hydrocortisone acetate, and cortisone acetate were also analyzed by the optimized method. The results indicated improvements in sensitivity ranging from 9.27 to 22.95% for the corticosteroids studied. The optimized method also decreased the assay time in relation to the official procedure by one-sixth.
采用两种独立的单纯形法对法定蓝四氮唑反应进行优化。通过同时改变药典方法的五个因素，即氢氧化四甲铵、蓝四氮唑和水的浓度，以及显色温度和时间，使固定量氢化可的松的净吸光度最大化。用初始单纯形法确定最佳响应，再用第二个单纯形法使在最佳条件下获得的空白吸光度最小化。还采用优化后的方法分析了一系列具有药学重要性的甾体：泼尼松、泼尼松龙、醋酸氢化可的松和醋酸可的松。结果表明，所研究的皮质类固醇的灵敏度提高了9.27%至22.95%。与法定方法相比，优化后的方法还将测定时间缩短了六分之一。
