Oteiza R M, Wooten R S, Kenner C T, Graham R E, Biehl E R
J Pharm Sci. 1977 Oct;66(10):1385-8. doi: 10.1002/jps.2600661008.
The kinetics of the reaction of blue tetrazolium with corticosteroids were investigated under pseudo-first-order conditions. The reaction rates for various corticosteroids were determined at various temperatures, and the enthalpy and entropy of activation for these compounds were determined. A mechanism is proposed in which an electron pair and a proton are transferred to blue tetrazolium from the anion formed by the action of tetramethylammonium hydroxide on the alpha-carbonyl moiety of the corticosteroid. The proposed mechanism is consistent with previous experimental results.
在准一级条件下研究了蓝四氮唑与皮质类固醇反应的动力学。测定了各种皮质类固醇在不同温度下的反应速率，并确定了这些化合物的活化焓和活化熵。提出了一种机制，其中电子对和质子从氢氧化四甲铵作用于皮质类固醇的α-羰基部分形成的阴离子转移到蓝四氮唑上。所提出的机制与先前的实验结果一致。
