Ayllón L, Silva M, Pérez-Bendito D
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
J Pharm Sci. 1994 Aug;83(8):1135-41. doi: 10.1002/jps.2600830813.
Two reaction-rate methods for the determination of corticosteroids at the micromolar level were developed. The methods are modifications of the widely used Blue Tetrazolium and Porter-Silber reactions which are employed for the automatic kinetic determination of corticosteroids using the continuous-addition-of-reagent (CAR) technique. The CAR technique avoided all technical problems typically associated with these reactions such as long incubation at high temperatures, high blank values, and turbidity. On the basis of its analytical figures of merit, the Blue Tetrazolium method was chosen; it allows corticosteroids to be determined at concentrations from 2.7 x 10(-6) to 1.9 x 10(-4) M at a rate of 25 samples/h (triplicate analyses) with an root mean square deviation of ca. 3%. The proposed method was evaluated in recovery experiments and analyses of commercially available formulations.
开发了两种用于测定微摩尔水平皮质类固醇的反应速率方法。这些方法是对广泛使用的蓝四氮唑和波特 - 西尔伯反应的改进，这两种反应采用连续加样（CAR）技术用于皮质类固醇的自动动力学测定。CAR技术避免了通常与这些反应相关的所有技术问题，如在高温下长时间孵育、高空白值和浑浊度。基于其分析性能指标，选择了蓝四氮唑法；它能够以每小时25个样品（重复分析）的速度测定浓度为2.7×10(-6)至1.9×10(-4) M的皮质类固醇，均方根偏差约为3%。所提出的方法在回收率实验和市售制剂分析中进行了评估。
