Byun Hye-Ryung, Ji Seong-Ryeong, Kang Jun-Gu, Choi Chang-Yong, Na Ki-Jeong, Kim Jong-Taek, Chae Joon-Seok
Laboratory of Veterinary Internal Medicine, BK21 FOUR Future Veterinary Medicine Leading Education and Research Centre, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Gwanak-ro 1, Gwanak-gu, Seoul 08826, Republic of Korea.
Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea.
One Health. 2024 Oct 10;19:100913. doi: 10.1016/j.onehlt.2024.100913. eCollection 2024 Dec.
Habitat loss of wildlife and increased human activities in their habitat provide more opportunities for human-wild animal contact. These artificial environments influence humans by facilitating the transmission of tick-borne pathogens. Therefore, we aimed to detect and understand circulating tick-borne pathogens in the natural environment by analyzing blood and spleen samples of wild animals admitted to wildlife rescue centers in the Republic of Korea. In total, 376 samples were collected from 355 rescued wild animals immediately after their arrival or death. After DNA deoxyribonucleic acid and RNA extractions, reverse transcription polymerase chain reaction (RT-PCR) and nested PCR were conducted to detect target tick-borne pathogens. This study detected six positive samples of severe fever with thrombocytopenia syndrome virus (SFTSV), 146 , 55 , 19 spp., 45 , and 4 . Among the positive samples, SFTSV was detected in one spleen sample from a Korean water deer, from which SFTSV was successfully isolated. After full genome sequencing, the L, M, and S segments all belonged to genotype B-3 and indicated 99.84 % ∼ 99.94 % similarity with SFTSV isolated from human serum. In conclusion, wild animals are potential reservoirs of tick-borne pathogens. Therefore, surveillance systems to prevent transmission among ticks, animals, and humans must be developed using the One Health concept.
野生动物栖息地的丧失以及人类在其栖息地活动的增加，为人类与野生动物的接触提供了更多机会。这些人工环境通过促进蜱传播病原体的传播而影响人类。因此，我们旨在通过分析被送往韩国野生动物救助中心的野生动物的血液和脾脏样本，来检测和了解自然环境中循环的蜱传播病原体。总共从355只获救野生动物到达或死亡后立即采集了376份样本。在进行脱氧核糖核酸（DNA）和核糖核酸（RNA）提取后，进行逆转录聚合酶链反应（RT-PCR）和巢式PCR以检测目标蜱传播病原体。本研究检测到6份发热伴血小板减少综合征病毒（SFTSV）阳性样本、146份……、55份……、19种……、45份……和4份……。在阳性样本中，从一只獐的脾脏样本中检测到SFTSV，并成功从中分离出SFTSV。全基因组测序后，L、M和S片段均属于B-3基因型，与从人血清中分离出的SFTSV的相似度为99.84%至99.94%。总之，野生动物是蜱传播病原体的潜在宿主。因此，必须采用“同一健康”理念建立监测系统，以防止蜱、动物和人类之间的传播。 （注：原文中部分“146, 55, 19 spp., 45, and 4.”表述不完整，无法准确翻译完整内容）
