Walker D H, Harrison A, Henderson F, Murphy F A
Am J Pathol. 1977 Feb;86(2):343-58.
Moribund guinea pigs infected with Richettsia rickettsii were examined by necropsy, histology, immunofluorescence, electron microscopy, and serology. Untreated animals died at 9 and 10 days after inoculation. Animals given saline subcutaneously survived from 1 to 4 days longer. Prolonged survival was accompanied by more severe lesions: scrotal necrosis; infarction of ears; and swollen, hemorrhagic footpads, epididymis, and cremaster muscle. Histopathologic examination demonstrated that acute, necrotizing vasculitis, perivascular hemorrhage, and focal necrosis were more extensive. Direct immunofluorescence indicated many more rickettsiae in endothelium and vascular wall of saline recipients. Ultrastructurally, typical rickettsiae were present focally in the cytoplasm of endothelial and vascular smooth muscle cells. Cytopathology in infected and adjacent cells included swelling, mitochondrial enlargement with decrease in matrix density and loss of cristae, and increased pinocytosis. In addition, treated animals had more cytonecrosis, thrombosis, extravascular fibrin deposition, prominent inflammatory cells with polymorphonuclear phagocytosis of rickettsiae, and antibody production.
对感染立氏立克次体的濒死豚鼠进行了尸检、组织学、免疫荧光、电子显微镜和血清学检查。未治疗的动物在接种后9天和10天死亡。皮下注射生理盐水的动物存活时间延长1至4天。存活时间延长伴随着更严重的病变：阴囊坏死；耳部梗死；脚垫、附睾和提睾肌肿胀、出血。组织病理学检查表明，急性坏死性血管炎、血管周围出血和局灶性坏死更为广泛。直接免疫荧光显示，接受生理盐水注射的动物的内皮和血管壁中有更多的立克次体。超微结构上，典型的立克次体局灶性存在于内皮细胞和血管平滑肌细胞的细胞质中。感染细胞和相邻细胞的细胞病理学变化包括肿胀、线粒体增大、基质密度降低和嵴消失，以及胞饮作用增加。此外，接受治疗的动物有更多的细胞坏死、血栓形成、血管外纤维蛋白沉积、有明显的炎症细胞以及对立克次体的多形核吞噬作用，并且产生了抗体。
