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使用基于培养的生物勘探策略从马赫尔草原土壤中鉴定出四种新的：新种、新种、新种和新种。

Identification of four novel isolated from machair grassland soil using a culture-based bioprospecting strategy: sp. nov., sp. nov., sp. nov. and sp. nov.

作者信息

Prole Josephine R, Allenby Nick, Manning David A C, Goodfellow Michael

机构信息

School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.

John Dawson Drug Discovery Centre, Sunderland University, Sunderland, UK.

出版信息

Int J Syst Evol Microbiol. 2025 Apr;75(4). doi: 10.1099/ijsem.0.006736.

DOI:10.1099/ijsem.0.006736
PMID:40208665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12281783/
Abstract

A culture-dependent bioprospecting strategy, based on the use of several selective isolation media, revealed the presence of relatively high numbers of streptomycete-like colonies from machair grassland soil, in which carbonate minerals dominate. Representatives were shown to be bioactive in primary and secondary antimicrobial screens conducted through standard plug assays. The comparison of the whole-genome sequences showed that four of the isolates were novel species in the genus , for which the names sp. nov. (=DSM 118365; =NCIMB 15554), sp. nov. (=DSM 118363; =NCIMB 15553), sp. nov. (=DSM 118364; =NCIMB 15555) and sp. nov. (=NCIMB 15556; =DSM 118366) are proposed. Genomes of the novel strains were found to be rich in biosynthetic gene clusters predicted to encode for diverse, specialized metabolites, notably antibiotics. They also contained stress-related genes that provided an insight into how streptomycetes cope with the prevailing conditions in machair grassland soils. It can be concluded that selective isolation and dereplication of streptomycetes from the unique machair habitat provides a practical way of isolating novel strains for ecological and biotechnological studies.

摘要

一种基于使用多种选择性分离培养基的依赖培养的生物勘探策略，揭示了在以碳酸盐矿物为主的马赫尔草原土壤中存在相对大量的链霉菌样菌落。通过标准菌块试验进行的一级和二级抗菌筛选表明，这些菌株具有生物活性。全基因组序列比较显示，其中四个分离株是该属的新物种，为此提出了新种名 sp. nov.（=DSM 118365；=NCIMB 15554）、 sp. nov.（=DSM 118363；=NCIMB 15553）、 sp. nov.（=DSM 118364；=NCIMB 15555）和 sp. nov.（=NCIMB 15556；=DSM 118366）。发现新菌株的基因组富含预测编码多种特殊代谢产物（尤其是抗生素）的生物合成基因簇。它们还包含与应激相关的基因，这些基因有助于深入了解链霉菌如何应对马赫尔草原土壤中的主要环境条件。可以得出结论，从独特的马赫尔生境中选择性分离链霉菌并进行重复排除，为生态和生物技术研究分离新的 菌株提供了一种切实可行的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/447e407a3bd8/ijsem-75-06736-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/3d41bfadc1a4/ijsem-75-06736-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/63829de92698/ijsem-75-06736-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/391d4e66a3db/ijsem-75-06736-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/14b7c5722bce/ijsem-75-06736-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/447e407a3bd8/ijsem-75-06736-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/3d41bfadc1a4/ijsem-75-06736-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/63829de92698/ijsem-75-06736-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/391d4e66a3db/ijsem-75-06736-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/14b7c5722bce/ijsem-75-06736-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fc2/12281783/447e407a3bd8/ijsem-75-06736-g005.jpg

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