Bielańska Patrycja, Golińska Patrycja, Romines Elaine, Rąbalski Łukasz, Węgrzyn Grzegorz
Department of Molecular Biology, Faculty of Biology, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Lwowska1, 87-100 Toruń, Poland.
Syst Appl Microbiol. 2025 Jul;48(4):126627. doi: 10.1016/j.syapm.2025.126627. Epub 2025 Jun 14.
In the present study the taxonomic position of two streptomycete strains, isolated from moonmilk in Szczelina Chochołowska cave located in Tatra Mountains in Poland, was established using a polyphasic taxonomic approach. Strains 2.9 and 4.24 have morphological characteristics and chemotaxonomic properties consistent with their classification in the genus Streptomyces. They are Gram-stain-positive filamentous bacteria which formed an extensively branched substrate mycelium and straight long chains of smooth surfaced spores. They contain LL-diaminopimelic acid, glucose and ribose in whole-organism hydrolysates, produce major proportions of straight, iso- and anteiso- fatty acids, hexa- and octa‑hydrogenated menaquinones with nine isoprene units and have a polar lipid pattern with phosphatidylethanolamine (diagnostic lipid). Strains 2.9 and 4.24 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing 100 % sequence similarity with Streptomyces stoeckheimensis DSM 116496ᵀ. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of strains 2.9 and 4.24 and their closely related phylogenomic neighbour were found to be 90.4 and 36.3 %, respectively, indicating that they belong to different genomic species. Consequently, based on the genomic, phylogenetic and associated phenotypic data it is proposed that strains 2.9 and 4.24 be assigned to the genus Streptomyces as Streptomyces tritrimontium sp. nov. with strain 2.9 (DSM 119353 = PCM 3548) as the type strain. The genomes of strains 2.9 and 4.24 contain relatively high number of biosynthetic gene clusters (28 and 27, respectively), some of which were discontinuously distributed, indicating ones predicted to express for novel specialized metabolites. Data acquired in the present study have also been used to emend the descriptions of Streptomyces manipurensis and Streptomyces nojiriensis.
在本研究中，采用多相分类学方法确定了从波兰塔特拉山脉Szczelina Chochołowska洞穴的月奶石中分离出的两株链霉菌菌株的分类地位。菌株2.9和4.24具有与链霉菌属分类一致的形态特征和化学分类学特性。它们是革兰氏染色阳性丝状细菌，形成广泛分支的基内菌丝体和平滑表面孢子的直长链。它们在全细胞水解物中含有LL-二氨基庚二酸、葡萄糖和核糖，产生主要比例的直链、异链和anteiso-脂肪酸、具有九个异戊二烯单元的六氢化和八氢化甲基萘醌，并且具有以磷脂酰乙醇胺（诊断性脂质）为特征的极性脂质模式。菌株2.9和4.24在链霉菌16S rRNA基因树中形成一个独特的分支，与Streptomyces stoeckheimensis DSM 116496ᵀ共享100%的序列相似性。从菌株2.9和4.24及其密切相关的系统发育邻居的全基因组序列计算出的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为90.4%和36.3%，表明它们属于不同的基因组物种。因此，基于基因组、系统发育和相关表型数据，建议将菌株2.9和4.24归入链霉菌属，命名为Streptomyces tritrimontium sp. nov.，以菌株2.9（DSM 119353 = PCM 3548）作为模式菌株。菌株2.9和4.24的基因组包含相对较多数量的生物合成基因簇（分别为28个和27个），其中一些是不连续分布的，表明其中一些预计会表达新的特殊代谢产物。本研究中获得的数据也已用于修订Streptomyces manipurensis和Streptomyces nojiriensis的描述。
