Bardajee Ghasem Rezanejade, Mahmoodian Hossein, Amiri Bita, Atashkadi Mojdeh
Department of Polymer and Materials Chemistry, Faculty of Chemistry and Petroleum Sciences, Shahid Beheshti University, 19839-63113 Tehran, Iran.
Department of Chemistry and Biochemistry, Chemistry Tech Company, 19369-34487 Tehran, Iran.
Langmuir. 2025 Apr 22;41(15):9601-9617. doi: 10.1021/acs.langmuir.4c04391. Epub 2025 Apr 10.
Developing a highly sensitive, selective, and biocompatible nanobiosensor for glucose monitoring remains a significant challenge in biomedical diagnostics. In this research, the design and synthesis of a highly sensitive and selective nanobiosensor for glucose detection were reported, utilizing cadmium telluride quantum dots (CdTe QDs) immobilized on a glucose-imprinted sodium alginate--poly(acrylic acid-copolymer-vinylphenylboronic acid) nanocomposite. The preparation method is notable for its safety, cost-effectiveness, and use of biocompatible materials. The nanobiosensor was synthesized by modifying CdTe QDs with a biopolymer chain comprising acrylic acid, sodium alginate, and vinylphenylboronic acid (VPBA), resulting in a glucose-sensitive and selective sensor with an average hydrodynamic diameter of 32 nm. Glucose detection was achieved through fluorescence quenching of CdTe QDs upon binding glucose molecules to VPBA moieties via cis-diol interactions, facilitating glucose sensing. A linear correlation was established between fluorescence intensity and glucose concentration, with a detection limit of 0.164 μg/mL. The sensor exhibited high specificity for glucose over potential interfering species, including various amino acids, fructose, lactic acid, and metal ions, even at concentrations 100 times higher than glucose. In practical applications, the sensor demonstrated high recovery rates ranging from 98.72 to 101.36% in human serum and urine samples, indicating its efficacy and specificity in complex biological matrices. The nanobiosensor also showed excellent repeatability and reproducibility with a relative standard deviation (RSD) of 1.25 and 1.38% for five replicates, respectively, and stability over 28 days with consistent fluorescence response. These results suggest that the glucose-imprinted Alg--P(AA--VPBA)/CdTe QDs nanobiosensor is a promising candidate for sensitive, selective, and glucose-monitoring applications.
开发一种用于葡萄糖监测的高灵敏度、高选择性且具有生物相容性的纳米生物传感器，仍然是生物医学诊断领域的一项重大挑战。在本研究中，报道了一种用于葡萄糖检测的高灵敏度和高选择性纳米生物传感器的设计与合成，该传感器利用固定在葡萄糖印迹海藻酸钠 - 聚（丙烯酸 - 共聚物 - 乙烯基苯硼酸）纳米复合材料上的碲化镉量子点（CdTe QDs）。该制备方法以其安全性、成本效益以及生物相容性材料的使用而著称。通过用包含丙烯酸、海藻酸钠和乙烯基苯硼酸（VPBA）的生物聚合物链修饰CdTe QDs来合成纳米生物传感器，得到了一种对葡萄糖敏感且具有选择性的传感器，其平均流体动力学直径为32 nm。当葡萄糖分子通过顺式二醇相互作用与VPBA部分结合时，CdTe QDs的荧光猝灭实现了葡萄糖检测，从而便于进行葡萄糖传感。在荧光强度和葡萄糖浓度之间建立了线性相关性，检测限为0.164 μg/mL。该传感器对葡萄糖表现出高于潜在干扰物质的高特异性，这些干扰物质包括各种氨基酸、果糖、乳酸和金属离子，即使在浓度比葡萄糖高100倍的情况下也是如此。在实际应用中，该传感器在人血清和尿液样本中的回收率高达98.72%至101.36%，表明其在复杂生物基质中的有效性和特异性。该纳米生物传感器还表现出出色的重复性和再现性，五次重复的相对标准偏差（RSD）分别为1.25%和1.38%，并且在28天内具有稳定的荧光响应。这些结果表明，葡萄糖印迹的Alg--P(AA--VPBA)/CdTe QDs纳米生物传感器是用于灵敏、选择性葡萄糖监测应用的有前途的候选者。
