Dumitru Corina Dana, Ilie Cornelia-Ioana, Neacsu Ionela Andreea, Motelica Ludmila, Oprea Ovidiu Cristian, Ripszky Alexandra, Pițuru Silviu Mirel, Voicu Bălașea Bianca, Marinescu Florica, Andronescu Ecaterina
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania.
National Research Center for Micro and Nanomaterials, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, Romania.
Int J Mol Sci. 2025 Jul 16;26(14):6809. doi: 10.3390/ijms26146809.
Honey, propolis or royal jelly are considered natural remedies with therapeutic properties since antiquity. Many papers explore the development of antimicrobial biomaterials based on individual bee products, but there is a lack of studies on their synergistic effects. Combining honey, propolis and royal jelly with silver nanoparticles in a biopolymer matrix offers a synergistic strategy to combat antibiotic-resistant bacterial infections. This approach supports progress in wound healing, soft tissue engineering and other domains where elimination of the microorganisms is needed like food packaging. In this study we have obtained antimicrobial films based on bee products and silver nanoparticles (AgNPs) incorporated in an alginate-chitosan blend. The novel biomaterials were analyzed by UV-Vis, fluorescence and FTIR spectroscopy or microscopy, SEM and thermal analysis. Antibacterial tests were conducted against both Gram-positive and Gram-negative bacteria, while the antifungal properties were tested against . The diameters for growth inhibition zones were up to 10 mm for bacterial strains and 8 mm for the fungal strain. Additionally, cytotoxicity assays were performed to evaluate the biocompatibility of the materials, the results indicating that the combination of honey, propolis, royal jelly and AgNPs does not produce synergistic toxicity.
自古以来，蜂蜜、蜂胶或蜂王浆就被视为具有治疗特性的天然药物。许多论文探讨了基于单一蜂产品的抗菌生物材料的开发，但缺乏对其协同作用的研究。将蜂蜜、蜂胶和蜂王浆与生物聚合物基质中的银纳米颗粒相结合，为对抗耐抗生素细菌感染提供了一种协同策略。这种方法有助于伤口愈合、软组织工程以及食品包装等其他需要消除微生物的领域取得进展。在本研究中，我们制备了基于蜂产品和掺入藻酸盐 - 壳聚糖共混物中的银纳米颗粒（AgNPs）的抗菌薄膜。通过紫外 - 可见光谱、荧光光谱、傅里叶变换红外光谱或显微镜、扫描电子显微镜和热分析对新型生物材料进行了分析。针对革兰氏阳性菌和革兰氏阴性菌进行了抗菌测试，同时针对真菌进行了抗真菌性能测试。细菌菌株的生长抑制圈直径可达10毫米，真菌菌株的生长抑制圈直径可达8毫米。此外，还进行了细胞毒性试验以评估材料的生物相容性，结果表明蜂蜜、蜂胶、蜂王浆和AgNPs的组合不会产生协同毒性。
